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Título: | Effect of purification of galactooligosaccharides derived from lactulose with Saccharomyces cerevisiae on their capacity to bind immune cell receptor Dectin-2 |
Autor: | Young, Ian D.; Montilla, Antonia CSIC ORCID ; Olano, Agustín CSIC ; Wittmann, Alexandra; Kawasaki, Norihito; Villamiel, Mar CSIC ORCID | Palabras clave: | Prebiotics Oligosaccharides Lactulose Mannoproteins Dectin-2 Saccharomyces |
Fecha de publicación: | 2019 | Editor: | Elsevier | Citación: | Food Research International 115: 10-15 (2019) | Resumen: | Lactulose-derived oligosaccharides (OsLu) are prebiotic galactooligosaccharides (GOS) beneficial for human health including immunomodulatory properties; however, the molecular mechanism is unclear. OsLu produced by enzymatic synthesis can be purified with Saccharomyces cerevisiae (OsLu-Sc). We show that this purification introduces yeast-derived proteins reactive to Dectin-2, an innate immune receptor for fungal polysaccharides. Using a cell-based bioassay, we tested the binding of OsLu and GOS samples to Dectin-2. While OsLu purified with active charcoal and commercial GOS failed to bind to Dectin-2, we found OsLu-Sc bound to this receptor. The carbohydrate-binding incompetent mutant of Dectin-2 failed to bind to OsLu-Sc. These data suggest that OsLu-Sc introduced carbohydrate ligands for Dectin-2. In accordance with this, proteomic analysis revealed OsLu-Sc contained S. cerevisiae-derived mannoproteins. Therefore, our data highlight the importance of the purification method for OsLu, which may positively affect the bioactivity of OsLu. Data are available via ProteomeXchange with identifier PXD010495. | Versión del editor: | https://doi.org/10.1016/j.foodres.2018.07.039 | URI: | http://hdl.handle.net/10261/174145 | DOI: | 10.1016/j.foodres.2018.07.039 | ISSN: | 0963-9969 | E-ISSN: | 1873-7145 |
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