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http://hdl.handle.net/10261/338494
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dc.contributor.author | Talens Perales, David | es_ES |
dc.contributor.author | Nicolau Sanus, María | es_ES |
dc.contributor.author | Marín Navarro, Julia | es_ES |
dc.contributor.author | Polaina Molina, Julio | es_ES |
dc.contributor.author | Daròs Arnau, José Antonio | es_ES |
dc.date.accessioned | 2023-11-07T11:24:59Z | - |
dc.date.available | 2023-11-07T11:24:59Z | - |
dc.date.issued | 2023-10-04 | - |
dc.identifier.citation | Current Research in Biotechnology 6: 100148 (2023) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/338494 | - |
dc.description.abstract | Glucose oxidase (GOX) catalyzes the FAD-dependent oxidation of α-D-glucose to D-gluconolactone with production of hydrogen peroxide. This enzyme encounters many biotechnological applications from glucose sensors to applications in food, pharma and textile industries. For this purpose, recombinant GOX versions, usually derived from Aspergillus niger, are produced in fermentation systems, frequently in filamentous fungi because other production platforms such as bacteria or yeast have rendered meager results. We wondered whether A. niger GOX, more specifically a mutant version with superior thermotolerant properties, could be efficiently produced in Nicotiana benthamiana plants. To this aim, we used a tobacco mosaic virus-derived vector that is inoculated into plant tissues using Agrobacterium tumefaciens. Results exhibited the efficient production of the recombinant GOX in plants and the facile downstream purification when the recombinant protein is targeted to the apoplast, the space between plasma membranes and cell walls. The plant-made recombinant GOX displayed excellent catalytic properties in broad pH and temperature conditions. In addition to establishing a new strategy to produce recombinant GOX in plants as a green alternative to traditional fungal fermentation, we further investigated the potential application of this protein as an ezybiotic. Results exhibited a remarkable bacteriocide activity against Escherichia coli and Staphylococcus aureus. | es_ES |
dc.description.sponsorship | This research was supported by Generalitat Valenciana, grant INNEST/2021/7 from Agència Valenciana de la Innovació, and from the Ministerio de Ciencia e Innovación (Spain), grant PID2020-114691RB-I00 from Agencia Estatal de Investigación. M.N.-S. is the recipient of a predoctoral contract (PRE2018-084771) from the Ministerio de Ciencia e Innovación. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114691RB-I00/ES/BIOTECNOLOGIA DE VIRUS DE PLANTAS: VECTORES VIRALES Y ESTRATEGIAS DE RESISTENCIA/ | es_ES |
dc.relation.ispartof | Current Research in Biotechnology | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Enzybiotic | es_ES |
dc.subject | Glucose oxidase | es_ES |
dc.subject | Plant biofactory | es_ES |
dc.subject | Viral vector | es_ES |
dc.title | Production in Nicotiana benthamiana of a thermotolerant glucose oxidase that shows enzybiotic activity against Escherichia coli and Staphylococcus aureus | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.crbiot.2023.100148 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.crbiot.2023.100148 | es_ES |
dc.identifier.e-issn | 2590-2628 | - |
dc.rights.license | http://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011033 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003359 | es_ES |
dc.contributor.orcid | #NODATA# | es_ES |
dc.contributor.orcid | #NODATA# | es_ES |
dc.contributor.orcid | #NODATA# | es_ES |
dc.contributor.orcid | #NODATA# | es_ES |
dc.contributor.orcid | #NODATA# | es_ES |
dc.identifier.scopus | 2-s2.0-85173258160 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85173258160 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.openairetype | artículo | - |
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CRB2023-Talens.pdf | Artículo principal | 3,18 MB | Adobe PDF | Visualizar/Abrir |
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