Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/257734
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development |
Autor: | Addante Moya, Luis G.; Abad Somovilla, Antonio; Abad Fuentes, Antonio CSIC ORCID; Agulló, Consuelo; Mercader Badia, Josep Vicent CSIC ORCID | Palabras clave: | ELISA Alternariol Antibody Hapten design Immunoassay Linker site |
Fecha de publicación: | 10-dic-2021 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Toxins 2021; 13(12): 883 | Resumen: | Immunochemical methods for mycotoxin analysis require antigens with well-defined structures and antibodies with outstanding binding properties. Immunoreagents for the mycotoxins alternariol and/or alternariol monomethyl ether have typically been obtained with chemically uncharacterized haptens, and antigen conjugates have most likely been prepared with mixtures of functionalized molecules. For the first time, total synthesis was performed, in the present study, to obtain two haptens with opposite linker attachment locations. The functionalized synthetic haptens were purified and deeply characterized by different spectrometric methods, allowing the preparation of bioconjugates with unequivocal structures. Direct and indirect competitive enzyme-linked immunosorbent assays, using homologous and heterologous conjugates, were employed to extensively evaluate the generated immunoreagents. Antibodies with high affinity were raised from conjugates of both haptens, and a structure-activity relationship between the synthetic haptens and the specificity of the generated antibodies could be established. These results pave the way for the development of novel highly sensitive immunoassays selective of one or two of these Alternaria mycotoxins. | Versión del editor: | https://doi.org/10.3390/toxins13120883 | URI: | http://hdl.handle.net/10261/257734 | DOI: | 10.3390/toxins13120883 | E-ISSN: | 2072-6651 |
Aparece en las colecciones: | (IATA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
toxins-13-00883-v2.pdf | Artículo principal | 1,3 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
3
checked on 30-abr-2024
SCOPUSTM
Citations
6
checked on 14-may-2024
WEB OF SCIENCETM
Citations
6
checked on 24-feb-2024
Page view(s)
49
checked on 16-may-2024
Download(s)
63
checked on 16-may-2024