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Título: | Identification of ACE-inhibitory peptides in different Spanish cheeses by tandem mass spectrometry |
Autor: | Gómez-Ruiz, José Ángel CSIC; Taborda, Gonzalo; Amigo, Lourdes CSIC ORCID ; Recio, Isidra CSIC ORCID ; Ramos González, María Mercedes CSIC | Palabras clave: | Bioactive peptides ACE-inhibitory activity HPLC-MS/MS PDO Spanish cheeses |
Fecha de publicación: | 21-mar-2006 | Editor: | Springer Nature | Citación: | European Food Research and Technology 223: 595- 601 (2006) | Resumen: | Different Spanish cheeses (Cabrales, Idiazábal, Roncal, Manchego, Mahón and a goat's milk cheese), made with diverse technologies and milk from different species, were studied for their angiotensin converting enzyme (ACE)-inhibitory activity. Among the water-soluble extract WSE<1000-Da of the different samples, Cabrales cheese was the most active with an inhibitory activity of 76.1%. On the contrary, Mahón cheese showed the lowest ACE-inhibitory index (56.6%). In order to identify the peptides involved in this activity, the WSE<1000-Da were analyzed by HPLC-MS/MS and off-line MS/MS. A total of 41 major peptides were identified in the different cheeses. Most of them derived from β-and αs1-casein. Several of these peptides were selected on the basis of the presence of proline as the C-terminal amino acid, and they were chemically synthesized. All peptides showed moderate or low ACE-inhibitory activity. Peptide DKIHP [β-CN f(47–51)], a sequence detected in all samples except Mahón cheese, showed the highest inhibitory activity with an IC50 value of 113.1 μM. | Versión del editor: | http://dx.doi.org/10.1007/s00217-005-0238-0 | URI: | http://hdl.handle.net/10261/256405 | DOI: | 10.1007/s00217-005-0238-0 | Identificadores: | doi: 10.1007/s00217-005-0238-0 issn: 1438-2377 e-issn: 1438-2385 |
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