Structural characteristics and biological activities of sulfated glycosaminoglycans extracted from shrimp by-products

Abstract

Sulfated glycosaminoglycans (SGSS) were extracted with enzymatic procedure from shrimp (Parapenaeus longirostris) byproducts and its biological properties were evaluated. The net yield of SGSS was found to be 7%. SGSS showed higher contents of sugar (97.66%) and uronic acid (46.48%) followed by sulfate (12%) and protein (1.37%). The monosaccharide composition revealed the presence of N-acetylgalactosamine, glucuronic, and galacturonic acids. The physicochemical characteristics were determined using XRD, DSC, and FTIR analysis. The antioxidant potency of SGSS was evaluated by several different in vitro systems, including DPPH free radical scavenging (IC50 = 1.05 mg/mL), β-Carotene bleaching inhibition (IC50 = 2.43 mg/mL), and iron-chelating capacity (IC50 = 0.17 mg/mL). ACE-inhibitory activity of SGSS was also investigated (IC50 = 1.74 mg/mL).