Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/339065
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Menero-Valdés, Paula | - |
dc.contributor.author | Lores-Padín, Ana | - |
dc.contributor.author | Fernández-Vega, Beatriz | - |
dc.contributor.author | Quarles Jr., C. Derrick | - |
dc.contributor.author | García, Montserrat | - |
dc.contributor.author | González-Iglesias, Héctor | - |
dc.contributor.author | Pereiro, Rosario | - |
dc.date.accessioned | 2023-11-16T11:00:20Z | - |
dc.date.available | 2023-11-16T11:00:20Z | - |
dc.date.issued | 2023-02 | - |
dc.identifier | doi: 10.1016/j.talanta.2022.123974 | - |
dc.identifier | issn: 0039-9140 | - |
dc.identifier | e-issn: 1873-3573 | - |
dc.identifier.citation | Talanta 253: 123974 (2023) | - |
dc.identifier.uri | http://hdl.handle.net/10261/339065 | - |
dc.description.abstract | Single cell-inductively coupled plasma-mass spectrometry (sc-ICP-MS) and laser ablation (LA)-ICP-MS have been complementary employed to develop a comprehensive study of APOE and claudin-1 expression in ARPE-19 cells submitted to a glucose treatment (100 mM, 48 h) that induces oxidative stress conditions. Results were compared with control cells. The determination of the two proteins by ICP-MS was sequentially carried out using specific immunoprobes labelled with IrNCs that offer a huge amplification (1760 ± 90 atoms of Ir on average). A novel sample introduction system, the microFAST Single Cell set-up, was employed for sc-ICP-MS analysis. This introduction system resulted in a cellular transport efficiency of 85 ± 9% for ARPE-19 cells (91 ± 5% using a PtNPs standard). After the proper immunocytochemistry protocol with the specific IrNCs immunoprobes in cell suspensions (sc-ICP-MS), the mass of APOE and claudin-1 in individual ARPE-19 cells was obtained. Average detection limits per cell by sc-ICP-MS were 0.02 fg of APOE and 3 ag of claudin-1. The results of sample analyses obtained by sc-ICP-MS were validated with commercial ELISA kits. The distribution of both target proteins in individual cells (fixated in the chamber wall) was unveiled by LA-ICP-MS. The high amplification provided by the IrNCs immunoprobes allowed the identification of APOE and claudin-1 within individual ARPE-19 cells. High resolution images were obtained using a laser spot of 2 × 2 μm. | - |
dc.description.sponsorship | This work was financially supported through projects PID2019- 107838RB-I00/Agencia Estatal de Investigacion ´ (AEI)/10.13039/ 501100011033) in Spain and AYUD/2021/51289 - FICYT. P. MeneroVald´es acknowledges the FPU Grant with Ref. FPU19/00556 (Ministry of Education, Spain). Authors would like to acknowledge David Clases (University of Graz, Austria) for the support with SPCaL software and the technical support provided by Servicios Científico-T´ecnicos of the University of Oviedo | - |
dc.format | application/pdf | - |
dc.language | eng | - |
dc.publisher | Elsevier BV | - |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107838RB-I00/ES/ESTUDIO DE LA METALOSTASIS EN ENFERMEDADES NEURODEGENERATIVAS ASOCIADAS AL ENVEJECIMIENTO EMPLEANDO NUEVAS ESTRATEGIAS ANALITICAS/ | - |
dc.relation.isversionof | Publisher's version | - |
dc.relation.isbasedon | Menero-Valdés, Paula; Lores-Padín, Ana; Fernández-Vega, Beatriz; Quarles Jr., C. Derrick;; García, Montserrat; González-Iglesias, Héctor; Pereiro, Rosario; 2023; Electronic Supplementary Material Determination and localization of specific proteins in individual ARPE-19 cells by single cell and laser ablation ICP-MS using iridium nanoclusters as label [Dataset]; Elsevier BV; https://doi.org/10.1016/j.talanta.2022.123974 | - |
dc.rights | openAccess | - |
dc.subject | Retinal pigment epithelial cells | - |
dc.subject | Iridium nanoclusters | - |
dc.subject | Metal-labelled immunoprobe | - |
dc.subject | sc-ICP-MS | - |
dc.subject | LA-ICP-MS | - |
dc.subject | Biomaging | - |
dc.title | Determination and localization of specific proteins in individual ARPE-19 cells by single cell and laser ablation ICP-MS using iridium nanoclusters as label | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.talanta.2022.123974 | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.talanta.2022.123974 | - |
dc.date.updated | 2023-11-16T11:00:21Z | - |
dc.rights.license | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.contributor.funder | Agencia Estatal de Investigación (España) | - |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | - |
dc.contributor.funder | Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología | - |
dc.contributor.funder | Ministerio de Universidades (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100008430 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011033 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (IPLA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
1-s2.0-S0039914022007706-main.pdf | 1,52 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
4
checked on 27-may-2024
Page view(s)
27
checked on 29-may-2024
Download(s)
20
checked on 29-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons