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| Título: | Supplemental material. CD4+ and CD8+ T-cell responses in bone marrow to fatty acids in high-fat diets |
Autor: | Medrano, Mayte; Lemus-Conejo, Ana CSIC ORCID CVN; López Martín, Sergio ; Millán-Linares, María del Carmen CSIC ORCID ; Rosillo, María Ángeles CSIC ORCID; Muñíz, Manuel CSIC ORCID; Calderon, Rocio; Jaramillo Carmona, Sara M.; Pérez-Simón, José A. CSIC ORCID; Pérez-Simón, José A. CSIC ORCID; Muriana, Francisco J. G. CSIC ORCID; Abia, Rocío CSIC ORCID | Fecha de publicación: | sep-2022 | Editor: | Elsevier | Citación: | Medrano, Mayte; Lemus-Conejo, Ana; López, Sergio; Millán-Linares, María del Carmen; Rosillo, Maria A; Muñíz, Manuel; Calderon, Rocio; Jaramillo Carmona, Sara M. ; Pérez-Simón, José A.; Muriana, Francisco J. G.; Abia, Rocío; 2022; Supplemental material. CD4+ and CD8+ T-cell responses in bone marrow to fatty acids in high-fat diets [Dataset]; Elsevier; http://dx.doi.org/10.1016/j.jnutbio.2022.109057 | Descripción: | Supplemental Table 1. Fatty acid composition in spleen cells of wild-type C57BL/6J mice at the end of 20-week dietary regimens. Supplemental Figure 1. An example of the gating strategy used to identify CD4+ (CD45+CD3+CD4+CD8–) and CD8+ (CD45+CD3+CD4–CD8+) T cells in spleen or bone marrow of wild-type C57BL/6J mice fed with the LFD and HFDs at the end of 20-week dietary regimens. SSC-A side scatter area, FSC-A forward scatter area, CD cluster of differentiation. Supplemental Figure 2. An example of the gating strategy used to identify CD3+ T cells and CD4+ and CD8+ T cell subsets in spleen cell suspensions of lean wild-type C57BL/6J mice after fatty acid treatment for 18 h. A similar gating strategy was used for bone marrow cell suspensions. SSC-A side scatter area, FSC-A forward scatter area, CD cluster of differentiation, 7-AAD 7-amino-actinomycin D. Supplemental Figure 3. CD3+ and CD4+ T cells in spleen cell suspensions of lean wild-type C57BL/6J mice treated with palmitic acid or oleic acid at indicated concentrations. The percentage relative to the negative control (cells without any treatment) of CD3+ and CD4+ T cells after the treatment of spleen cell suspensions with low-endotoxin fatty acid-free BSA control, BSA-palmitic acid complex (25-300 M) (A, D) or BSA-oleic acid complex (25-300 M) (B, E) for 18 h. The comparative of palmitic and oleic acids at 300 M is also shown (C, F). The data are presented as the mean (bars) and individual points ± SD values (n = 6), and those marked with different letters are significantly different (p<0.05). Differences between groups were assessed by one-way ANOVA with Tukey’s post hoc test or, in the case of variance heterogeneity, by Kruskal-Wallis with Bonferroni correction. CD cluster of differentiation, PA palmitic acid, OA oleic acid. Supplemental Figure 4. CD8+ T cells in spleen and CD3+ T cells in bone marrow (BM) cell suspensions of lean wild-type C57BL/6J mice treated with palmitic acid or oleic acid at indicated concentrations. The percentage relative to the negative control (cells without any treatment) of CD8+ T cells (spleen) and CD3+ T cells (BM) after the treatment of each cell suspension with low-endotoxin fatty acid-free BSA control, BSA-palmitic acid complex (25-300 M) (A, D) or BSA-oleic acid complex (25-300 M) (B, E) for 18 h. The comparative of palmitic and oleic acids at 300 M is also shown (C, F). The data are presented as the mean (bars) and individual points ± SD values (n = 6), and those marked with different letters are significantly different (p<0.05). Differences between groups were assessed by one-way ANOVA with Tukey’s post hoc test or, in the case of variance heterogeneity, by Kruskal-Wallis with Bonferroni correction. CD cluster of differentiation, PA palmitic acid, OA oleic acid. Supplemental Figure 5. CD4+ and CD8+ T cells in bone marrow (BM) cell suspensions of lean wild-type C57BL/6J mice treated with palmitic acid or oleic acid at indicated concentrations. The percentage relative to the negative control (cells without any treatment) of CD4+ and CD8+ T cells after the treatment of BM cell suspensions with low-endotoxin fatty acid-free BSA control, BSA-palmitic acid complex (300 M) (A, D) or BSA-oleic acid complex (300 M) (B, E) for 18 h. The comparative of palmitic and oleic acids at 300 M is also shown (C, F). The data are presented as the mean (bars) and individual points ± SD values (n = 6), and those marked with different letters are significantly different (p<0.05). Differences between groups were assessed by one-way ANOVA with Tukey’s post hoc test or, in the case of variance heterogeneity, by Kruskal-Wallis with Bonferroni correction. CD cluster of differentiation, PA palmitic acid, OA oleic acid. | Versión del editor: | http://dx.doi.org/10.1016/j.jnutbio.2022.109057 | URI: | http://hdl.handle.net/10261/331501 | DOI: | 10.1016/j.jnutbio.2022.109057 | Referencias: | Medrano, Mayte; Lemus-Conejo, Ana; López, Sergio; Millán-Linares, María del Carmen; Rosillo, Maria A; Muñíz, Manuel; Calderon, Rocio; Jaramillo Carmona, Sara M. ; Pérez-Simón, José A.; Muriana, Francisco J. G.; Abia, Rocío. CD4+ and CD8+ T-cell responses in bone marrow to fatty acids in high-fat diets. http://dx.doi.org/10.1016/j.jnutbio.2022.109057 . http://hdl.handle.net/10261/282847 |
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| high-fat diets_suppl.docx | 1,37 MB | Microsoft Word XML | Visualizar/Abrir | |
| README.txt | 3,49 kB | Text | Visualizar/Abrir |
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