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dc.contributor.author | Mejías-Ortiz, Miguel | - |
dc.contributor.author | Mencher-Beltrán, Ana | - |
dc.contributor.author | Morales, Pilar | - |
dc.contributor.author | Tronchoni, Jordi | - |
dc.contributor.author | González García, Ramón | - |
dc.date.accessioned | 2023-03-22T16:40:02Z | - |
dc.date.available | 2023-03-22T16:40:02Z | - |
dc.date.issued | 2022-04-07 | - |
dc.identifier.citation | 3rd Iberoamerican Congress on Biotechnology (2022) | - |
dc.identifier.uri | http://hdl.handle.net/10261/303924 | - |
dc.description | Trabajo presentado en el 3rd Iberoamerican Congress on Biotechnology, celebrado en Braga (Portugal), del 7 al 9 de abril de 2022 | - |
dc.description.abstract | Malolactic fermentation is essential for the quality of red wines and some other wine styles. This process consists in the decarboxylation of L-malic acid to L-lactic acid, increasing the pH of wine and releasing compounds with sensory impact [1]. Spontaneous malolactic fermentation is often driven by Oenococcus oeni, and commercial starters for this purpose are also often of this species. The microbial interactions during wine fermentation are a growing field of interest due to the new inoculation strategies in winemaking processes. Among other interaction mechanisms, extracellular vesicles (EVs) might play a relevant role on it. Determination of the protein cargo of the EVs is a first approach to understand how biological processes can be affected in by the EVs. In this work, the production of extracellular vesicles by O. oeni is reported for the first time. The protein content of O. oeni EVs was characterised by proteomic analysis. It shares some characteristics with the recently described protein content of Lactiplantibacillus plantarum EVs [3], a bacterial species also capable of performing malolactic fermentation of wine. EVs produced by O. oeni are similar in size to other bacterial EVs described previously, and the proteomic analysis shows an enrichment in ribosomal and integral membrane proteins, which are in accordance with other bacterial and eukaryotic EV cargo. Considering ubiquity of O. oeni in winemaking, this work will help in future studies on microbial interactions relevant for this industry. [1] Genomic variations of Oenococcus oeni strains and the potential to impact on malolactic fermentation and aroma compounds in wine, Bartowsky, E.J., Borneman, A.R., Appl. Microbiol. Biotechnol., 92., 441¿447 (2011). [2] Extracellular vesicles in food biotechnology, Morales, P., Mencher, A., Tronchoni, J., Gonzalez, R., Microb. Biotechnol., 14., 8¿11 (2021). [3] Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line, Bajic, S.S., Cañas, M.-A., Tolinacki, M., Badia, J., Sánchez, B., Golic, N., Margolles, A., Baldomá, L., Ruas-Madiedo, P., Sci. Rep., 10., 21829 (2020). | - |
dc.description.sponsorship | This work was funded by the Spanish Government through grants PID2019-105159RB-I00 funded by MCIN/AEI/10.13039/501100011033 and BES-2016-077557 and PRE2020-093420 funded by MCIN/AEI/10.13039/501100011033 and by ¿ESF Investing in your future¿ (training contracts for AM and MM). JT was funded by FGCSIC by the COMFUTURO program. | - |
dc.format | application/pdf | - |
dc.language | eng | - |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105159RB-I00/ES/VESICULAS EXTRACELULARES DE LEVADURAS EN ENOLOGIA/ | - |
dc.relation | info:eu-repo/grantAgreement/MINECO//AGL2015-63629-R/ES/ANALISIS MOLECULAR DE LAS INTERACCIONES ENTRE CULTIVOS INICIADORES DURANTE LA VINIFICACION/ | - |
dc.relation | info:eu-repo/grantAgreement/AEI//PRE2020-093420 | - |
dc.rights | openAccess | - |
dc.title | Protein content of the Oenococcus oeni extracellular vesicles-enriched fraction | - |
dc.type | póster de congreso | - |
dc.date.updated | 2023-03-22T16:40:02Z | - |
dc.contributor.funder | Agencia Estatal de Investigación (España) | - |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Fundación General CSIC | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011033 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100006003 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6670 | es_ES |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | póster de congreso | - |
Aparece en las colecciones: | (ICVV) Comunicaciones congresos |
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