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Chemical synthesis of a fully active transcriptional repressor protein

AuthorsSolar, Gloria del ; Albericio, Fernando; Eritja Casadellà, Ramón ; Espinosa, Manuel
KeywordsAffinity purfication
Gel retardation assays
In vitro transcription
Issue Date24-May-1994
PublisherNational Academy of Sciences (U.S.)
CitationProceedings of the National Academy of Sciences of the USA 91(11): 5178-5182 (1994)
AbstractPlasmid pLS1-encoded 45-amino acid transcriptional repressor CopG (formerly RepA) has been chemically synthesized. A one-step purification of the synthetic protein has been developed, which yields high levels of pure protein with low or no contamination of truncated products. We have compared some properties of the chemical CopG protein with those of the biologically purified CopG. The two proteins were indistinguishable in (i) their ability to generate specific protein-DNA complexes, (ii) their capacity to protect a restriction site included within the CopG DNA target, and (iii) in their in vitro capacity to specifically repress synthesis of copG mRNA.
Description5 pages, 5 figures.-- PMID: 8197204 [PubMed].-- PMCID: PMC43955.
Publisher version (URL)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC43955
Appears in Collections:(IQAC) Artículos
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