2024-03-28T17:26:40Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/274582020-10-20T10:55:00Zcom_10261_5063com_10261_5col_10261_5066
00925njm 22002777a 4500
dc
Solar, Gloria del
author
Albericio, Fernando
author
Eritja Casadellà, Ramón
author
Espinosa, Manuel
author
1994-05-24
Plasmid pLS1-encoded 45-amino acid transcriptional repressor CopG (formerly RepA) has been chemically synthesized. A one-step purification of the synthetic protein has been developed, which yields high levels of pure protein with low or no contamination of truncated products. We have compared some properties of the chemical CopG protein with those of the biologically purified CopG. The two proteins were indistinguishable in (i) their ability to generate specific protein-DNA complexes, (ii) their capacity to protect a restriction site included within the CopG DNA target, and (iii) in their in vitro capacity to specifically repress synthesis of copG mRNA.
Proceedings of the National Academy of Sciences of the USA 91(11): 5178-5182 (1994)
0027-8424
http://hdl.handle.net/10261/27458
1091-6490
Affinity purification
Gel retardation assays
In vitro transcription
Chemical synthesis of a fully active transcriptional repressor protein