English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/208079
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Optimization of pyrogallol autoxidation conditions and its application in evaluation of superoxide anion radical scavenging capacity for four antioxidants

AuthorsZhang, Quing An; Wang, Xi; Song, Yun; Fan, Xue-Hui; García-Martín, Juan Francisco
Issue Date2016
PublisherAOAC International
CitationJournal of AOAC International 99: 504- 511 (2016)
AbstractIn this study, some factors influencing pyrogallol autoxidation, including EDTA, temperature, and solvent, were systematically investigated to improve its feasibility in the evaluation of antioxidants for the first time. Subsequently, the improved pyrogallol autoxidation conditions were used to assess the superoxide anion scavenging activity (SASA) of four commonly used antioxidants, namely, ascorbic acid, rutin, catechin, and gallic acid, by both the reaction rate method and the terminated method. The results indicate that pyrogallol autoxidation could be successfully used to determine the antioxidant capacity of ascorbic acid and rutin, which correspondingly suggests the feasibility of its use to measure the superoxide anion radical scavenging activity of polysaccharides and flavonols, because these compounds have a similar basic structural unit as ascorbic acid and rutin, respectively. Unexpectedly, however, pyrogallol autoxidation cannot be used to evaluate the SASA of catechin and gallic acid, although their good antioxidant capacity was confirmed by the 1,1-diphenyl-2-picrylhydrazyl assay. Together, these results suggest the importance of noting the conditions used for pyrogallol autoxidation when assessing the SASA of targeted compounds.
Publisher version (URL)http://dx.doi.org/10.5740/jaoacint.15-0223
URIhttp://hdl.handle.net/10261/208079
Identifiersdoi: 10.5740/jaoacint.15-0223
issn: 1060-3271
Appears in Collections:(IG) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.