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Título: | Use of green fluorescent protein to monitor cell envelope stress in lactococcus lactis |
Autor: | Campelo, Ana B. CSIC; Rodríguez González, Ana CSIC ORCID; Martínez Fernández, Beatriz CSIC ORCID | Palabras clave: | GFP detection Stress Lactococcus lactis |
Fecha de publicación: | feb-2010 | Editor: | American Society for Microbiology | Citación: | Applied and Environmental Microbiology 76(3): 978-981 (2010) | Resumen: | A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfpuv gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR Copyright © 2010, American Society for Microbiology. All Rights Reserved. | URI: | http://hdl.handle.net/10261/51413 | DOI: | 10.1128/AEM.02177-09 | Identificadores: | doi: 10.1128/AEM.02177-09 issn: 0099-2240 |
Aparece en las colecciones: | (IPLA) Artículos |
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GFP.pdf | 329,38 kB | Adobe PDF | Visualizar/Abrir |
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