Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/50267COMPARTIR / EXPORTAR:
 SHARE
 CORE
BASE
|
|
| Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
| Título: | Mechanisms underlying differential food allergy response to heated egg |
Autor: | Martos, Gustavo CSIC ORCID; López-Expósito, Iván CSIC | Palabras clave: | Egg allergy Ovalbumin Ovomucoid Heat treatment Heating Gastrointestinal digestion |
Fecha de publicación: | abr-2011 | Editor: | Elsevier | Citación: | Journal of Allergy and Clinical Immunology 127(4): 990-997.e2 (2011) | Resumen: | [Background]: Egg white proteins are usually subjected to heating, making them edible for the majority of children with egg allergy. [Objective]: We sought to investigate the underlying mechanisms responsible for the reduced allergenicity displayed by heat-treated egg white allergens. [Methods]: C3H/HeJ mice were orally sensitized with ovalbumin (OVA) or ovomucoid and challenged with native or heated proteins to evaluate their allergenicity. Immunoreactivity was assessed by immunoblotting using sera from children with egg allergy. In vitro gastrointestinal digestion of native and heated OVA and ovomucoid was studied by SDS-PAGE and liquid chromatography. Intestinal uptake of intact native and heated OVA and ovomucoid by human intestinal epithelial (Caco-2) cells was investigated. Rat basophil leukemia cells passively sensitized with mouse serum and human basophils passively sensitized with serum from children with egg allergy were used to assess the effector cell activation by heated, digested, and transported OVA and ovomucoid. [Results]: Heated OVA and ovomucoid did not induce symptoms of anaphylaxis in sensitized mice when administered orally. Heating did not completely destroy IgE-binding capacity of OVA or ovomucoid but enhanced in vitro digestibility of OVA. Digestion of both OVA and ovomucoid diminished mediator release in rat basophil leukemia assay and basophil activation. Heating of allergens prevented transport across human intestinal epithelial cells in a form capable of triggering basophil activation or T-cell activation. Conclusion Heat treatment reduces allergenicity of OVA and ovomucoid. This is partially a result of the enhanced gastrointestinal digestibility of heated OVA and the inability of heated OVA or ovomucoid to be absorbed in a form capable of triggering basophils | Descripción: | 10 páginas, 7 figuras.-- et al. | Versión del editor: | http://dx.doi.org/10.1016/j.jaci.2011.01.057 | URI: | http://hdl.handle.net/10261/50267 | DOI: | 10.1016/j.jaci.2011.01.057 | ISSN: | 0091-6749 | E-ISSN: | 1097-6825 |
| Aparece en las colecciones: | (CIAL) Artículos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| restringido.pdf | 21,67 kB | Adobe PDF |  Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
26
checked on 16-may-2024
SCOPUSTM
Citations
123
checked on 19-may-2024
WEB OF SCIENCETM
Citations
81
checked on 21-feb-2024
Page view(s)
327
checked on 23-may-2024
Download(s)
202
checked on 23-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.