Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/47157
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Carbon nanotube composite peptide-based biosensors as putative diagnostic tools for rheumatoid arthritis |
Autor: | Gracia Villa, María de; Jiménez-Jorquera, Cecilia CSIC ORCID CVN ; Haro Villar, Isabel CSIC ORCID ; Gómara Elena, María José CSIC ORCID; Sanmartí, Raimon; Fernández Sánchez, César CSIC ORCID ; Mendoza Gómez, Ernest CSIC ORCID | Palabras clave: | Rheumatoid arthritis Immunosensor Carbon nanotube–polystyrene composite Amperometry |
Fecha de publicación: | 2011 | Editor: | Elsevier | Citación: | Biosensors and Bioelectronics | Resumen: | This work reports on the fabrication and performance of a simple amperometric immunosensor device to be potentially used for the detection of serum anti-citrullinated peptide antibodies (ACPAs), which are specific for rheumatoid arthritis (RA) autoimmune disease. Sera of RA patients contain antibodies to different citrullinated peptides and proteins such as fibrin or filaggrin. Herein, a chimeric fibrin–filaggrin synthetic peptide (CFFCP1) was used as a recognition element anchored to the surface of a multiwalled carbon nanotube–polystyrene (MWCNT–PS) based electrochemical transducer. The transducer fabrication process is described in detail together with its successful electrochemical performance in terms of repeatability and reproducibility of the corresponding amperometric response. The resulting immunosensor approach was initially tested in sera of rabbits previously inoculated with the synthetic peptide and eventually applied to the detection of ACPAs in human sera. A comparative study was carried out using control serum from a blood donor, which demonstrated the selectivity of the immunosensor response and its sensitivity for the detection of anti-CFFCP1 antibodies present in RA patients. | Versión del editor: | http://dx.doi.org/10.1016/j.bios.2011.06.026 | URI: | http://hdl.handle.net/10261/47157 | DOI: | 10.1016/j.bios.2011.06.026 | ISSN: | 0956-5663 | E-ISSN: | 1873-4235 |
Aparece en las colecciones: | (IQAC) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
38
checked on 05-may-2024
WEB OF SCIENCETM
Citations
32
checked on 17-feb-2024
Page view(s)
452
checked on 07-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.