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Título: | Identification of RNAi hypoallergic bread wheat lines for wheat-dependent exercise-induced anaphylaxis patients |
Autor: | Guzmán-López, María H. CSIC; Ruipérez, Violeta CSIC ORCID; Marín-Sanz, Miriam CSIC; Ojeda-Fernández, Isabel; Ojeda-Fernández, Pedro; Garrote, José Antonio CSIC ORCID CVN; Arranz, Eduardo CSIC ORCID; Barro Losada, Francisco CSIC ORCID | Palabras clave: | RNAi Gluten IgE Wheat Wheat-dependent exercise-induced anaphylaxis (WDEIA) Reverse-phase high-performance liquid chromatography (RP-HPLC) Non-gluten proteins (NGPs) |
Fecha de publicación: | 2023 | Editor: | Frontiers Media | Citación: | Frontiers in Nutrition 10: 1319888 (2023) | Resumen: | Wheat-dependent exercise-induced anaphylaxis (WDEIA) is one of the most severe forms of wheat allergy. It occurs in patients when they exercise after ingesting wheat-containing foods. Nowadays, the only possible alternative for WDEIA patients is to avoid such foods. This study investigated the potential of six RNA of interference (RNAi) wheat lines with low-prolamin content as alternatives for WDEIA patients. For that purpose, a high performance-liquid chromatography (HPLC) analysis was performed to evaluate differences in gluten protein fractions among these lines. Next, western blots were conducted to measure the immunoglobulin E (IgE) reactivity to wheat proteins in sera from five WDEIA patients. Additionally, monoclonal antibodies (moAb) recognition sites and the IgE binding sites were searched in all peptides identified by LC-MS/MS after protein digestion. The results showed a 61.4%–81.2% reduction in the gliadin content of the RNAi lines, accompanied by an increase in their high-molecular weight (HMW) glutenin content compared to the wild type bread wheat line (WT). In all cases, the reduction in gliadin content correlated with a decrease in IgE reactivity observed in the sera of WDEIA patients, highlighting the E82 and H320 lines. These two RNAi lines exhibited a ≤90% reduction in IgE reactivity. This reduction could be attributed to an absence of IgE binding sites associated with α- and ω5-gliadins, which were present in the WT. Overall, these lines offer a potential alternative for foodstuff for individuals with WDEIA. | Versión del editor: | https://doi.org/10.3389/fnut.2023.1319888 | URI: | http://hdl.handle.net/10261/353228 | DOI: | 10.3389/fnut.2023.1319888 | E-ISSN: | 2296-861X |
Aparece en las colecciones: | (IBGM) Artículos (IAS) Artículos |
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RNAi-hypoallergic_Guzman_Art_2023.pdf | 1,52 MB | Adobe PDF | Visualizar/Abrir |
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