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Título: | Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
Autor: | Rodríguez Pulido, Miguel Ramón CSIC ORCID; Calvo Pinilla, Eva María; Polo, Miryam; Saiz Calahorra, Juan Carlos; Fernández González, Raúl; Pericuesta Camacho, Eva; Gutiérrez-Adán, Alfonso CSIC ORCID ; Sobrino Castelló, Francisco CSIC ORCID; Martín-Acebes, M. A.; Sáiz, Margarita CSIC ORCID | Palabras clave: | COVID-19 RNA-based therapy SARS-CoV-2 Antiviral immunity Coronaviruses Foot-and-mouth disease virus (FMDV) Non-coding RNA Type-I IFN |
Fecha de publicación: | 29-mar-2023 | Editor: | Frontiers Media | Citación: | Frontiers in Immunology 14: e1166725 (2023) | Resumen: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of a potentially severe respiratory disease, the coronavirus disease 2019 (COVID-19), an ongoing pandemic with limited therapeutic options. Here, we assessed the anti-coronavirus activity of synthetic RNAs mimicking specific domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs). These molecules are known to exert broad-spectrum antiviral activity in cell culture, mice and pigs effectively triggering the host innate immune response. The ncRNAs showed potent antiviral activity against SARS-CoV-2 after transfection in human intestinal Caco-2 and lung epithelium Calu-3 2B4 cells. When the in vivo efficacy of the FMDV ncRNAs was assessed in K18-hACE2 mice, administration of naked ncRNA before intranasal SARS-CoV-2 infection significantly decreased the viral load and the levels of pro-inflammatory cytokines in the lungs compared with untreated infected mice. The ncRNAs were also highly efficacious when assayed against common human HCoV-229E and porcine transmissible gastroenteritis virus (TGEV) in hepatocyte-derived Huh-7 and swine testis ST cells, respectively. These results are a proof of concept of the pan-coronavirus antiviral activity of the FMDV ncRNAs including human and animal divergent coronaviruses and potentially enhance our ability to fight future emerging variants. | Descripción: | 12 Pág. | Versión del editor: | https://doi.org/10.3389/fimmu.2023.1166725 | URI: | http://hdl.handle.net/10261/330229 | DOI: | 10.3389/fimmu.2023.1166725 | E-ISSN: | 1664-3224 |
Aparece en las colecciones: | (INIA) Artículos (PTI Salud Global) Colección Especial COVID-19 |
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