Determination of polar compounds, polymerized and oxidized triacylglycerols, and diacylglycerols in oils and fats: Results of collaborative studies and the standardized method (Technical report)

Abstract

A description is given of the development by collaborative study of twostandardized methods for the determination of polar compounds in oils and fats byadsorption chromatography using silica minicolumns, and for quantification ofpolymerized triacylglycerols, oxidized triacylglycerols, and diacylglycerols inpolar compounds by high-performance size-exclusion chromatography. The firstprocedure is sensitive, allowing savings in time, solvents, and reagents as com-pared to the previous determination (Standard Method 2.507), while the second isvery rapid, giving a detailed information on the main groups of compounds in fatsand oils associated with hydrolysis, oxidation, and thermal polymerization. Bothmethods are useful for the analysis of used frying fats as well as for the analysisof virgin or refined oils.