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Effects of butyrate feed supplementation on gilthead sea bream (Sparus aurata) growth performance and intestinal health: A transcriptomic approach

AuthorsPérez-Sánchez, Jaume ; Bermejo-Nogales, Azucena ; Grammes F.; Pérez-Cordón, G. ; Øverland M.; Calduch-Giner, Josep A. ; Mallo, J. J.; Sitjà-Bobadilla, Ariadna ; Ballester-Lozano, Gabriel F.
Issue DateNov-2013
CitationAquaculture conference >To the next 40 years of sustainable global aquaculture> (2013)
AbstractThe aim of the present study was to evaluate the effects of a short fatty acid, the commercial butyrate product (BP-70, ®Norel), on gilthead sea bream performance and intestinal health. Juvenile fish of 25 g initial body weight were distributed in 90-L triplicate tanks/group (15 fish/tank). Fish were fed plant protein-based diets with 20% fish meal and 35% plant oil at the expense of fish oil, and with increasing levels of BP-70 (0%, 0.2%, 0.4%, 0.8%) for 9 weeks. No significant effects of butyrate supplementation were found on growth rates, feed conversion ratio, or retention of N and lipids. No effects were found on hepatosomatic index or viscerosomatic index, but the gut index (fish weight/intestine length) was progressively and significantly increased with butyrate supplementation. Butyrate also increased plasma glucose levels and liver glycogen depots, which highly supports a sparing effect of butyrate on the utilization of glucose as a metabolic fuel. A PCR-array of 90 genes was used to characterize the intestinal gene expression pattern of the two extreme groups (0%, 0.8% diets). Genes were selected as markers of intestine cell proliferation and differentiation, intestinal architecture and permeability, enterocyte mass and epithelia damage, intestinal immune-surveillance and mitochondria activity. The differentially expressed genes of all these categories showed that butyrate supplementation clearly induced a healthy intestine condition. In particular, components of the Hedgehog, bone morphogenic protein and Notch signalling pathways were up-regulated in butyrate treated fish, which would orchestrate a complex regulatory network promoting intestine cell differentiation rather than stem cell proliferation. This agrees with the lowered expression of the proliferating cell nuclear antigen (PCNA), as evidenced by RT-PCR and immunocytochemistry. Butyrate also improved the intestine barrier function, up-regulating the expression of several components of tight junctions (occludin, claudin 12, claudin 15, tight junction protein ZO-1, and coxsackievirus and adenovirus receptor homolog), and altered the expression of nuclear-encoded mitochondrial genes, up regulating the expression of master transcription factors, mitochondrial protein translocases and oxidative enzymes of the tricarboxylic acid cycle, and down-regulated the expression of mitochondrial molecular chaperones of the Hsp 10 family. This expression pattern is indicative of a mitochondrial phenotype with a ¿high power¿ activity and a low risk of oxidative stress. In addition, butyrate supplementation altered the expression of interleukin 7, nucleotide-binding protein oligomerization domain-containing protein 1, vimentin, macrophage mannose receptor 1 and C-C motif chemokine 20, leading to an anti-inflammatory gene expression pattern. Therefore, butyrate supplementation as a whole is a very promising approach to improve the health condition of gilthead sea bream intestine.
DescriptionPoster presentado en la Aquaculture conference "To the next 40 years of sustainable global aquaculture" celebrada en Gran Canaria del 3 al 7 de noviembre de 2013
Appears in Collections:(IATS) Comunicaciones congresos
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