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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/74026
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dc.contributor.authorGarcía-Cayuela, Tomás-
dc.contributor.authorGómez de Cadiñanos, Luz P.-
dc.contributor.authorMohedano Bonillo, Mari Luz-
dc.contributor.authorFernández de Palencia, P.-
dc.contributor.authorBoden, Daniel-
dc.contributor.authorWells, Jerry-
dc.contributor.authorPeláez, Carmen-
dc.contributor.authorLópez García, Paloma-
dc.contributor.authorRequena, Teresa-
dc.date.accessioned2013-04-10T18:02:21Z-
dc.date.available2013-04-10T18:02:21Z-
dc.date.issued2012-
dc.identifier.citationApplied Microbiology and Biotechnology 96(1): 171-181 (2012)-
dc.identifier.issn0175-7598-
dc.identifier.urihttp://hdl.handle.net/10261/74026-
dc.description29 p.- 4 tab.-2 fig.-
dc.description.abstractFluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.-
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (grants AGL2009-12998-C03-01, AGL2009- 13361-C02-02, and CSD2007-00063 Consolider Ingenio 2010 FUN-CFOOD), the Comunidad de Madrid (grant ALIBIRD P2009/AGR-1469), and the European Union VII framework program (Initial Training Network, grant no. 238490). We thank Dr. Stephen Elson for the critical reading of the manuscript. We are grateful to M. Angeles Corrales for her technical assistance.-
dc.language.isoeng-
dc.publisherSpringer Nature-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/238490-
dc.rightsopenAccess-
dc.titleFluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli-
dc.typeartículo-
dc.identifier.doi10.1007/s00253-012-4087-z-
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s00253-012-4087-z-
dc.identifier.e-issn1432-0614-
dc.date.updated2013-04-10T18:02:21Z-
dc.description.versionPeer Reviewed-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderComunidad de Madrid-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.openairetypeartículo-
item.fulltextWith Fulltext-
item.languageiso639-1en-
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