Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/61277
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Size and conformational features of ErbB2 and ErbB3 receptors: A TEM and DLS comparative study |
Autor: | Vicente-Alique, Ernesto CSIC; Núñez-Ramírez, Rafael CSIC ; Vega, Juan Francisco CSIC ORCID; Hu, P.; Martínez-Salazar, Javier CSIC ORCID | Fecha de publicación: | 2011 | Editor: | Springer Nature | Citación: | European Biophysics Journal 40: 835- 842 (2011) | Resumen: | ErbB2 and ErbB3 receptors belong to the epidermal growth factor receptor family. The members of this family are able to form homo- and heterodimers that trigger diverse downstream signalling concerned to multiple cellular events. In the absence of a ligand, ErbB3 adopts a characteristic tethered conformation, which differs from ErbB2 extended conformation. In this work, transmission electron microscopy (TEM) and dynamic light scattering (DLS) have been used to characterize the conformational features and the size of ErBb2 and ErbB3 receptors. Two main objectives are presented. The first one is to evaluate the use of TEM as a tool for structural studies for this family of receptors. The low molecular weight of these proteins represents a challenging purpose for TEM studies. The other one is to search for a relationship between the results obtained by TEM and those obtained for the hydrodynamic size measured by DLS. This comparison has allowed us to identify the conformational differences of the receptors and to anticipate the use of these experimental techniques for the study of the ligand activated heterodimerization, a process related to a significant number of human malignancies. © 2011 European Biophysical Societies' Association. | URI: | http://hdl.handle.net/10261/61277 | DOI: | 10.1007/s00249-011-0699-y | Identificadores: | doi: 10.1007/s00249-011-0699-y issn: 0175-7571 |
Aparece en las colecciones: | (CFMAC-IEM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
10
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
11
checked on 27-feb-2024
Page view(s)
272
checked on 24-abr-2024
Download(s)
140
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.