Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/310643
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Novel chimeric proteins mimicking SARS-CoV-2 spike epitopes with broad inhibitory activity |
Autor: | Cano-Muñoz, Mario; Polo-Megías, Daniel; Cámara-Artigas, Ana; Gavira Gallardo, J. A. CSIC ORCID ; López-Rodríguez, María J.; Laumond, Géraldine; Schmidt, Sylvie; Demiselle, Julien; Bahram, Seiamak; Moog, Christiane; Conejero-Lara, Francisco | Palabras clave: | COVID-19 Fusion inhibitor Vaccine |
Fecha de publicación: | 1-dic-2022 | Editor: | Elsevier | Citación: | International Journal of Biological Macromolecules 222: 2467-2478 (2022) | Resumen: | SARS-CoV-2 spike (S) protein mediates virus attachment to the cells and fusion between viral and cell membranes. Membrane fusion is driven by mutual interaction between the highly conserved heptad-repeat regions 1 and 2 (HR1 and HR2) of the S2 subunit of the spike. For this reason, these S2 regions are interesting therapeutic targets for COVID-19. Although HR1 and HR2 have been described as transiently exposed during the fusion process, no significant antibody responses against these S2 regions have been reported. Here we designed chimeric proteins that imitate highly stable HR1 helical trimers and strongly bind to HR2. The proteins have broad inhibitory activity against WT B.1 and BA.1 viruses. Sera from COVID-19 convalescent donors showed significant levels of reactive antibodies (IgG and IgA) against the HR1 mimetic proteins, whereas these antibody responses were absent in sera from uninfected donors. Moreover, both inhibitory activity and antigenicity of the proteins correlate positively with their structural stability but not with the number of amino acid changes in their HR1 sequences, indicating a conformational and conserved nature of the involved epitopes. Our results reveal previously undetected spike epitopes that may guide the design of new robust COVID-19 vaccines and therapies. | Versión del editor: | http://dx.doi.org/10.1016/j.ijbiomac.2022.10.031 | URI: | http://hdl.handle.net/10261/310643 | DOI: | 10.1016/j.ijbiomac.2022.10.031 | Identificadores: | doi: 10.1016/j.ijbiomac.2022.10.031 issn: 0141-8130 |
Aparece en las colecciones: | (IACT) Artículos (PTI Salud Global) Colección Especial COVID-19 |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Novel chimeric proteins mimicking.pdf | 4,72 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
5
checked on 10-may-2024
WEB OF SCIENCETM
Citations
3
checked on 29-feb-2024
Page view(s)
41
checked on 16-may-2024
Download(s)
45
checked on 16-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons