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Título: | Multiple mechanisms driving F-actin-dependent transport of organelles to and from secretory sites in bovine chromaffin cells |
Autor: | Gimenez-Molina, Yolanda CSIC ORCID; Villanueva, José CSIC ORCID; Francés, María del Mar CSIC; Viniegra, Salvador CSIC ORCID; Gutiérrez, Luis M. CSIC ORCID | Fecha de publicación: | 2018 | Editor: | Frontiers Media | Citación: | Frontiers in Cellular Neuroscience 12: 344 (2018) | Resumen: | Neuroendocrine chromaffin cells represent an excellent model to study the molecular mechanisms associated with the exo-endocytotic cycle of neurotransmitter release. In this study, EGFP-Lifeact and confocal microscopy has been used to analyze the re-organization of the cortical F-actin cytoskeleton associated to organelle transport during secretion with unprecedented detail. In these cells secretory events accumulate in temperature-sensitive and myosin II-dependent F-actin expansions and retractions affecting specific regions of the sub-membrane space. Interestingly, not only vesicles but also mitochondria are transported toward the plasmalemma during these expansions. Simultaneously, we found F-actin cytoskeletal retraction withdraws vesicles from the sub-plasmalemmal space, forming novel empty internal spaces into which organelles can be transported. In addition to these well-coordinated, F-actin-myosin II dependent processes that drive the transport of the majority of vesicles, fast transport of chromaffin vesicles was observed, albeit less frequently, which used F-actin comet tails nucleated from the granular membrane. Thus, upon cell stimulation F-actin structures use diverse mechanisms to transport organelles to and from the membrane during the exo-endocytotic cycle taking place in specific areas of cell periphery. | Versión del editor: | https://doi.org/10.3389/fncel.2018.00344 | URI: | http://hdl.handle.net/10261/309231 | DOI: | 10.3389/fncel.2018.00344 | E-ISSN: | 1662-5102 |
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