Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/305713
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Response regulator PorX coordinates oligonucleotide signalling and gene expression to control the secretion of virulence factors |
Autor: | Schmitz, Claus A. CSIC ORCID ; Madej, Mariusz; Nowakowska, Zuzanna; Cuppari, Anna CSIC; Jacula, Anna; Ksiazek, Miroslaw; Mikruta, Katarzyna; Wisniewski, Jerzy; Pudelko-Malik, Natalia; Saran, Anshu; Zeytuni, Natalie; Mlynarz, Piotr; Lamont, Richard J; Usón, Isabel CSIC ORCID ; Siksnys, Virginijus; Potempa, Jan; Sola, María | Fecha de publicación: | 28-nov-2022 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research 50(21): 12558-12577 (2022) | Resumen: | The PglZ family of proteins belongs to the alkaline phosphatase superfamily, which consists of metallohydrolases with limited sequence identity but similar metal-coordination architectures in otherwise divergent active sites. Proteins with a well-defined PglZ domain are ubiquitous among prokaryotes as essential components of BREX phage defence systems and two-component systems (TCSs). Whereas other members of the alkaline phosphatase superfamily are well characterized, the activity, structure and biological function of PglZ family proteins remain unclear. We therefore investigated the structure and function of PorX, an orphan response regulator of the Porphyromonas gingivalis TCS containing a putative PglZ effector domain. The crystal structure of PorX revealed a canonical receiver domain, a helical bundle, and an unprecedented PglZ domain, similar to the general organization of the phylogenetically related BREX-PglZ proteins. The PglZ domain of PorX features an active site cleft suitable for large substrates. An extensive search for substrates revealed that PorX is a phosphodiesterase that acts on cyclic and linear oligonucleotides, including signalling molecules such as cyclic oligoadenylates. These results, combined with mutagenesis, biophysical and enzymatic analysis, suggest that PorX coordinates oligonucleotide signalling pathways and indirectly regulates gene expression to control the secretion of virulence factors. | Versión del editor: | http://dx.doi.org/10.1093/nar/gkac1103 | URI: | http://hdl.handle.net/10261/305713 | DOI: | 10.1093/nar/gkac1103 | Identificadores: | doi: 10.1093/nar/gkac1103 issn: 1362-4962 |
Aparece en las colecciones: | (IBMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Response regulator PorX_Schmitz_PV_Art2022.pdf | 8,89 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
1
checked on 04-may-2024
Page view(s)
46
checked on 15-may-2024
Download(s)
59
checked on 15-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons