Partial characterization of jumbo squid skin pigment extract and its antioxidant potential in a marine oil system

Abstract

Pigment compounds were extracted from jumbo squid (Dosidicus gigas) skin with an acid-ethanol solvent (JSE). Freeze-dried JSE was characterized with respect to solubility in different solvents, absorption UV–VIS, and FT-IR spectra, and tested for its radical scavenging activity against ABTS and oxygen radical absorbance capacity (ORAC). The potential ability of JSE for inhibiting oxidation of cod liver oil (CLO) was also determined by monitoring dienes, trienes, peroxide value (PV), thiobarbutiric acid (TBA), and polyene index (PI) in samples stored at 15, 25, and 50°C for 12 days. Concentrations of 0, 0.1, 0.5, 1, and 2% JSE were added to CLO. The yield of JSE was 8.8 mg/g freeze-dried skin. Solubility behavior, UV–Vis, and FT-IR spectra of JSE suggests that this pigment extract might belong to the ommochrome family. Moreover, a characteristic xanthommatin peak (1740 cm−1) was observed. JSE exhibited scavenging activity on ABTS•+ radical and in the ORAC assay. After storage PV and TBA increased, whereas PI decreased mainly in the control treatment. The addition of JSE delayed lipid oxidation in CLO during the first 8 days of storage at 50°C. JSE was identified as promising source of antioxidants to retard fish lipid oxidation.