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Título: | Down-regulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection |
Autor: | Cisneros, Adriana E.; Lisón, Purificación; Campos, Laura CSIC ORCID; López-Tubau, Joan Manel CSIC; Altabella, Teresa CSIC ORCID; Ferrer, Albert; Daròs Arnau, José Antonio CSIC ORCID ; Carbonell, Alberto CSIC ORCID | Palabras clave: | Solanum lycopersicum Artificial microRNA Hypersusceptibility Sterol glycosyltransferase Viroid |
Fecha de publicación: | 13-mar-2023 | Editor: | Oxford University Press | Citación: | Journal of Experimental Botany 74(5): 1564-1578 (2023) | Resumen: | Potato spindle tuber viroid (PSTVd) is a plant pathogen naturally infecting economically important crops such as tomato (Solanum lycopersicum). Here, we aimed to engineer tomato plants highly resistant to PSTVd and developed several S. lycopersicum lines expressing an artificial microRNA (amiRNA) against PSTVd (amiR-PSTVd). Infectivity assays revealed that amiR-PSTVd-expressing lines were not resistant but instead hypersusceptible to the viroid. A combination of phenotypic, molecular, and metabolic analyses of amiRNA-expressing lines non-inoculated with the viroid revealed that amiR-PSTVd was accidentally silencing the tomato STEROL GLYCOSYLTRANSFERASE 1 (SlSGT1) gene, which caused late developmental and reproductive defects such as leaf epinasty, dwarfism, or reduced fruit size. Importantly, two independent transgenic tomato lines each expressing a different amiRNA specifically designed to target SlSGT1 were also hypersusceptible to PSTVd, thus demonstrating that down-regulation of SlSGT1 was responsible for the viroid-hypersusceptibility phenotype. Our results highlight the role of sterol glycosyltransferases in proper plant development and indicate that the imbalance of sterol glycosylation levels favors viroid infection, most likely by facilitating viroid movement. | Versión del editor: | https://doi.org/10.1093/jxb/erac361 | URI: | http://hdl.handle.net/10261/304779 | DOI: | 10.1093/jxb/erac361 |
Aparece en las colecciones: | (CRAG) Artículos (IBMCP) Artículos |
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Cisneros et al. 2022 Journal of Experimental Botany.pdf | 3,03 MB | Adobe PDF | Visualizar/Abrir |
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