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Título : High-Q dynamic force microscopy in liquid and its application to living cells
Autor : Tamayo de Miguel, Francisco Javier ; Humphris, A. D. L.; Owen, R. J.; Miles, M. J.
Fecha de publicación : jul-2001
Editor: Biophysical Society
Citación : Biophys J. 2001 July; 81(1): 526–537.
Resumen: We present a new dynamic force microscopy technique for imaging in liquids in the piconewton regime. The low quality factor (Q) of the cantilever is increased up to three orders of magnitude by the implementation of a positive feedback control. The technique also includes a phase-locked loop unit to track the resonance of the cantilever. Experiments and computer simulations indicate that the tip-sample forces are below 100 pN, about two orders of magnitude lower than in conventional tapping mode atomic force microscopy. Furthermore, the spectroscopic ability is greatly enhanced. Either the phase shift or the resonant frequency shows a high sensitivity to variations in either the energy dissipation or conservative interactions between the tip and the sample, respectively. The potential of this technique is demonstrated by imaging living cells.
Versión del editor: http://www.biophysj.org/cgi/content/full/81/1/526
URI : http://hdl.handle.net/10261/2814
ISSN: 0006-3495
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