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Título: | Application of enzyme-linked immunosorbent assay (ELISA) for the study of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia): II. Impacts of Perkinsus olseni on clam reproduction |
Autor: | Park, Kyoung; Figueras Huerta, Antonio CSIC ORCID ; Choi, Kwang-Sik | Palabras clave: | Reproductive output Ruditapes philippinarum Perkinsus olseni ELIS Gametogenesis Korea |
Fecha de publicación: | 2006 | Editor: | Elsevier | Citación: | Aquaculture 251(2-4): 182-191 (2004) | Resumen: | We investigated the effects of infection by the protozoan Perkinsus olseni on the reproduction of female Manila clams, Ruditapes philippinarum, from a population in Gomso Bay, Korea. The reproductive effort of the clams was assessed by ELISA using a clam egg-specific antibody and was expressed as a weight-based gonadosomatic index (GSI). The number of Perkinsus infecting each clam was estimated from the gills using Ray's fluid thioglycollate medium (RFTM) along with a NaOH digestion assay. We found that reproductive effort was negatively correlated with the intensity of the Perkinsus infection: more heavily infected clams produced fewer eggs during the spawning period from May to August. Frequency of spawning was also negatively correlated with the level of Perkinsus infection; heavily infected clams (HIC) exhibited a single spawning pulse in late July, whereas lightly infected clams (LIC) showed three spawning peaks in mid-May, late July, and late August. Egg production of HIC was only 30–75% of LIC during spawning. The level of total protein in LIC was also higher year round than in HIC. In conclusion, our investigation demonstrates that a high level of Perkinsus infection affects spawning frequency and reduces egg production, which may have long-term impacts on clam recruitment and population growth | Descripción: | 10 pages, 5 figures, 2 tables | Versión del editor: | https://doi.org/10.1016/j.aquaculture.2005.06.003 | URI: | http://hdl.handle.net/10261/277512 | DOI: | 10.1016/j.aquaculture.2005.06.003 | ISSN: | 0044-8486 |
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