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Título: | Subcellular localization of glutelin-2 in maize (Zea mays L.) endosperm |
Autor: | Ludevid, M. Dolors CSIC ; Torrent, Margarita CSIC ; Martínez-Izquierdo, José Antonio CSIC; Puigdomènech, Pere CSIC; Palau, Jaume | Fecha de publicación: | 1984 | Editor: | Springer Nature | Citación: | Plant Molecular Biology 3: 227-234 (1984) | Resumen: | Accumulation of the 28 KD protein of the glutelin-(G2) fraction was followed in developing maize endosperm, using sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and peak integration of scanned gels. 28 KD glutelin-2 could already be observed from 15 days after pollination and its accumulates reached a plateau during the second half of the development period. The process of biosynthesis of 28 KD glutelin-2 and zeins occurs in a parallel way. Subcellular fractions obtained from linear sucrose gradient centrifugation of developing maize endosperms were analyzed by SDS-PAGE and immunoblotting using a serum reacting against glutelin-2 and 14 KD Z2. Glutelin-2 was found to be present in the protein bodies when subcellular fractionation was carried out without dithiothreitol (DTT). The presence of a reducing agent causes the elution of glutelin-2 from protein bodies. Immunocytochemical labelling using the protein A-colloidal gold technique in protein bodies incubated with anti-G2 IgG revealed that G2 is located mainly in the periphery of protein bodies. These results are interpreted as indicating a structural role for glutelins in protein bodies. | Versión del editor: | https://doi.org/10.1007/BF00029658 | URI: | http://hdl.handle.net/10261/274628 | DOI: | 10.1007/BF00029658 | ISSN: | 0167-4412 |
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