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Título: | The use of conformationally rigid nucleoside probes to study the role of sugar pucker and nucleobase orientation in the thrombin binding aptamer |
Autor: | Saneyoshi, Hisao; Mazzini, Stefania; Aviñó, Anna CSIC ORCID; Portella, Guillem; González, Carlos CSIC ; Orozco, Modesto; Márquez, Víctor E.; Eritja Casadellà, Ramón CSIC ORCID | Fecha de publicación: | 27-sep-2009 | Editor: | Oxford University Press | Citación: | Nucleic Acids Symposium Series 53(1): 109-110 (2009) | Resumen: | Thrombin binding aptamers (TBAs) incorporating North-/South-deoxyguanosines built on the rigid bicyclo[3.1.0]hexane template were synthesized. Individual 2'-deoxyguanosines at positions dG14 and dG15 of the aptamer were replaced by these analogues where the North/anti and South/syn conformational states were confined. The substitution at position 14 with a locked South/syn-dG nucleoside produced an aptamer with the same stability and global structure as the innate, unmodified one. Replacing position 15 with the same South/syndG nucleoside induced a strong destabilization of the aptamer, while the antipodal North/anti-dG nucleoside was less destabilizing. Remarkably, the insertion of a North/anti-dG nucleoside at position 14, where both pseudosugar conformation and glycosyl torsion angle are opposite with respect to the native structure, led to the complete disruption of the G-tetraplex structure as detected by NMR and confirmed by extensive molecular dynamics simulations. | Descripción: | 2 pages, 1 figure, 1 table.-- PMID: 19749284 [PubMed]. | Versión del editor: | http://dx.doi.org/10.1093/nass/nrp055 | URI: | http://hdl.handle.net/10261/27394 | DOI: | 10.1093/nass/nrp055 | ISSN: | 1746-8272 (Online) |
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