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dc.contributor.authorDíez, Diego-
dc.contributor.authorMorte, Beatriz-
dc.contributor.authorBernal, Juan-
dc.date.accessioned2022-03-28T13:10:11Z-
dc.date.available2022-03-28T13:10:11Z-
dc.date.issued2021-
dc.identifier.citationThyroid: official journal of the American Thyroid Association 31: 1577-1588 (2021)-
dc.identifier.issn1050-7256-
dc.identifier.urihttp://hdl.handle.net/10261/265487-
dc.description© Diego Diez et al., 2021; Published by Mary Ann Liebert, Inc.-
dc.description.abstract[Background]: Thyroid hormones are crucial for brain development, acting through the thyroid hormone nuclear receptors (TR)α1 and β to control gene expression. Triiodothyronine (T3), the receptor-ligand, is transported into the brain from the blood by the monocarboxylate transporter 8 (MCT8). Another source of brain T3 is from the local deiodination of thyroxine (T4) by type 2 deiodinase (DIO2). While these mechanisms are very similar in mice and humans, important species-specific differences confound our understanding of disease using mouse models. To fill this knowledge gap on thyroid hormone action in the human fetal brain, we analyzed the expression of transporters, DIO2, and TRs, which we call thyroid hormone effectors, at single-cell resolution. [Methods]: We analyzed publicly available single-cell transcriptome data sets of isolated cerebral cortex neural cells from three different studies, with expression data from 393 to almost 40,000 cells. We generated Uniform Manifold Approximation and Projection scatterplots and cell clusters to identify differentially expressed genes between clusters, and correlated their gene signatures with the expression of thyroid effectors. [Results]: The radial glia, mainly the outer radial glia, and astrocytes coexpress SLCO1C1 and DIO2, indicating close cooperation between the T4 transporter OATP1C1 and DIO2 in local T3 formation. Strikingly, THRB was mainly present in two classes of interneurons: a majority expressing CALB2/calretinin, from the caudal ganglionic eminence, and in somatostatin-expressing interneurons from the medial ganglionic eminence. By contrast, many cell types express SLC16A2 and THRA. [Conclusions]:SLCO1C1 and DIO2 coexpression in the outer radial glia, the universal stem cell of the cerebral cortex, highlights the likely importance of brain-generated T3 in neurogenesis. The unique expression of THRB in discrete subsets of interneurons is a novel finding whose pathophysiological meaning deserves further investigation.-
dc.description.sponsorshipThis study was supported by grant #SAF2017-86342-R (Programa Estatal de Investigacion, Desarrollo e Innovacio´n Orientada a los Retos de la Sociedad), and the Center for Biomedical Research on Rare Diseases, Instituto de Salud Carlos III. The cost of this publication has been paid in part by FEDER funds.-
dc.languageeng-
dc.publisherMary Ann Liebert-
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-86342-R/ES/MECANISMOS PATOGENICOS EN LA DEFICIENCIA DE MCT8: UN ENFOQUE MULTIDISCIPLINAR HACIA TRATAMIENTOS BASADOS EN EL CONOCIMIENTO/-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.subjectFetus-
dc.subjectInterneurons-
dc.subjectMCT8-
dc.subjectNuclear receptors-
dc.subjectOATP1C1-
dc.subjectType 2 deiodinas-
dc.titleSingle-Cell Transcriptome Profiling of Thyroid Hormone Effectors in the Human Fetal Neocortex: Expression of SLCO1C1, DIO2, and THRB in Specific Cell Types-
dc.typeartículo-
dc.identifier.doi10.1089/thy.2021.0057-
dc.relation.publisherversionhttp://dx.doi.org/10.1089/thy.2021.0057-
dc.identifier.e-issn1557-9077-
dc.date.updated2022-03-28T13:10:11Z-
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)-
dc.contributor.funderAgencia Estatal de Investigación (España)-
dc.contributor.funderCentro de Investigación Biomédica en Red de Enfermedades Infecciosas (España)-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderEuropean Commission-
dc.relation.csicSí-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairetypeartículo-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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