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Título: | Experimental evolution reveals a genetic basis for membrane-associated virus release |
Autor: | Bou, Juan-Vicente; Sanjuán, Rafael CSIC ORCID | Palabras clave: | Viral transmission Directed evolution Ultra-deep sequencing Virus–membrane interactions Enterovirus |
Fecha de publicación: | 2021 | Editor: | Oxford University Press | Citación: | Molecular Biology and Evolution 38(2): 358–367 (2021) | Resumen: | Many animal viruses replicate and are released from cells in close association to membranes. However, whether this is a passive process or is controlled by the virus remains poorly understood. Importantly, the genetic basis and evolvability of membrane-associated viral shedding have not been investigated. To address this, we performed a directed evolution experiment using coxsackievirus B3, a model enterovirus, in which we repeatedly selected the free-virion or the fast-sedimenting membrane-associated viral subpopulations. The virus responded to this selection regime by reproducibly fixing a series of mutations that altered the extent of membrane-associated viral shedding, as revealed by full-genome ultra-deep sequencing. Specifically, using site-directed mutagenesis, we showed that substitution N63H in the viral capsid protein VP3 reduced the ratio of membrane-associated to free viral particles by 2 orders of magnitude. These findings open new avenues for understanding the mechanisms and implications of membrane-associated viral transmission. | Versión del editor: | https://doi.org/10.1093/molbev/msaa208 | URI: | http://hdl.handle.net/10261/262101 | DOI: | 10.1093/molbev/msaa208 | E-ISSN: | 1537-1719 |
Aparece en las colecciones: | (I2SysBio) Artículos |
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experimenrelease.pdf | 574,13 kB | Adobe PDF | Visualizar/Abrir |
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