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http://hdl.handle.net/10261/262101
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Bou, Juan-Vicente | es_ES |
dc.contributor.author | Sanjuán, Rafael | es_ES |
dc.date.accessioned | 2022-02-25T12:34:03Z | - |
dc.date.available | 2022-02-25T12:34:03Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Molecular Biology and Evolution 38(2): 358–367 (2021) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/262101 | - |
dc.description.abstract | Many animal viruses replicate and are released from cells in close association to membranes. However, whether this is a passive process or is controlled by the virus remains poorly understood. Importantly, the genetic basis and evolvability of membrane-associated viral shedding have not been investigated. To address this, we performed a directed evolution experiment using coxsackievirus B3, a model enterovirus, in which we repeatedly selected the free-virion or the fast-sedimenting membrane-associated viral subpopulations. The virus responded to this selection regime by reproducibly fixing a series of mutations that altered the extent of membrane-associated viral shedding, as revealed by full-genome ultra-deep sequencing. Specifically, using site-directed mutagenesis, we showed that substitution N63H in the viral capsid protein VP3 reduced the ratio of membrane-associated to free viral particles by 2 orders of magnitude. These findings open new avenues for understanding the mechanisms and implications of membrane-associated viral transmission. | es_ES |
dc.description.sponsorship | This work was funded by the European Research Council (Grant No. 724519 Vis-aVis) and the Generalitat Valenciana (Prometeo Grant No. 2016/122). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/724519 | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Viral transmission | es_ES |
dc.subject | Directed evolution | es_ES |
dc.subject | Ultra-deep sequencing | es_ES |
dc.subject | Virus–membrane interactions | es_ES |
dc.subject | Enterovirus | es_ES |
dc.title | Experimental evolution reveals a genetic basis for membrane-associated virus release | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1093/molbev/msaa208 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1093/molbev/msaa208 | es_ES |
dc.identifier.e-issn | 1537-1719 | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | European Research Council | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000781 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003359 | es_ES |
dc.identifier.pmid | 32810259 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (I2SysBio) Artículos |
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experimenrelease.pdf | 574,13 kB | Adobe PDF | Visualizar/Abrir |
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