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dc.contributor.author | Cameselle, José C. | - |
dc.contributor.author | Costas, María J. | - |
dc.contributor.author | Günther Sillero, María A. | - |
dc.contributor.author | Sillero, Antonio | - |
dc.date.accessioned | 2010-04-29T12:40:50Z | - |
dc.date.available | 2010-04-29T12:40:50Z | - |
dc.date.issued | 1982-02-01 | - |
dc.identifier.citation | Biochemical Journal 201(2): 405-410 (1982) | en_US |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | http://hdl.handle.net/10261/23744 | - |
dc.description | 6 pages, 1 figure, 2 tables.-- Los autores pertenecen al antiguo Instituto de Enzimología del CSIC. | en_US |
dc.description.abstract | The occurrence and distribution of bis-(5'-guanosyl) tetraphosphatase activity towards dinucleoside tetraphosphates between the 27 000 g supernatant and sedimented fraction were studied in liver, kidney, brain, muscle and intestinal mucosa from rat. The p1p4-bis-(5'-guanosyl) tetraphosphate-hydrolysing activities found in total homogenates were 0.77, 1.44, 0.39, 0.36 and 2.14 units (mumol/min)/g respectively. The activities found in the 27000 g-sedimented fractions were 74, 49, 11, 4 and 96% of those present in the homogenates respectively. The properties of the soluble enzymes were investigated. All of them have low Km values for p1p4-bis-(5'-guanosyl) tetraphosphate (from 2 to 50 microM), are competitively inhibited by guanosine 5'-tetraphosphate with K1 values from 10 to 160 nM, have molecular weights of about 21 000, require Mg2+ or Mn2+ and are inhibited by Ca2+. These properties show that bis-(5'-guanosyl) tetraphosphatase (EC 3.6.1.17), an enzyme previously characterized in Artemia salina and rat liver [Warner & Finamore (1965) Biochemistry 4, 1568-1575; Vallejo, Sillero & Sillero (1974) Biochim, Biophys. Acta 358, 117-125; Lobatón, Vallejo, Sillero & Sillero (1975) Eur. J. Biochem. 50, 495-501], is present in all the rat tissues examined. The inhibition of the enzyme by Ca2+ could be related to the effect of p1p4-bis-(5'-adenosyl) tetraphosphate as a trigger of DNA synthesis [Grummt, Waltl, Jantzen, Hamprecht, Huebscher & Kuenzle (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 6081-6085]. | en_US |
dc.description.sponsorship | This investigation was supported by grants from Diputación Foral and Caja de Ahorros Provincial de Alava, Ayuntamiento and Caja de Ahorros Municipal de Vitoria, Fondo Nacional para el Desarrollo de la Investigación Científica and Fundación Juan March. | en_US |
dc.format.extent | 938512 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Biochemical Society | en_US |
dc.rights | openAccess | en_US |
dc.title | Bis-(5'-guanosyl) tetraphosphatase in rat tissues | en_US |
dc.type | artículo | en_US |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1163657/ | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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