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http://hdl.handle.net/10261/208554
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Jamshaid, Talha | es_ES |
dc.contributor.author | Tenório-Neto, Ernandes Taveira | es_ES |
dc.contributor.author | Baraket, Abdellatif | es_ES |
dc.contributor.author | Lebaz, Noureddine | es_ES |
dc.contributor.author | Elaissari, Abdelhamid | es_ES |
dc.contributor.author | Sanchís Villariz, Ana | es_ES |
dc.contributor.author | Salvador, Juan Pablo | es_ES |
dc.contributor.author | Marco, María Pilar | es_ES |
dc.contributor.author | Bausells, Joan | es_ES |
dc.contributor.author | Errachid, Abdelhamid | es_ES |
dc.contributor.author | Zine, Nadia | es_ES |
dc.date.accessioned | 2020-04-22T08:02:38Z | - |
dc.date.available | 2020-04-22T08:02:38Z | - |
dc.date.issued | 2020-04-21 | - |
dc.identifier.citation | Biosensors 10 (4): 43 (2020) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/208554 | - |
dc.description.abstract | In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. The analyte was quantified by competitive detection with SA2BSA immobilized on the WE toward a mixture of Ab155 antibody (with fixed concentration) and sulfapyridine. In order to obtain a highly sensitive biosensor, Ab155 was immobilized onto magnetic latex nanoparticles surface to create a 3D architecture (Ab-MLNp). Using electrochemical impedance spectroscopy (EIS), we investigated the influence of the Ab-MLNp on the sensitivity of our approach. The optimized system was analyzed, as competitive assay, with different concentrations of sulfapyridine (40 µM, 4 µM, and 2 nM) and with phosphate buffer solution. From data fitting calculations and graphs, it was observed that the EIS showed more linearity when Ab-MLNp was used. This result indicates that the magnetic latex nanoparticles increased the sensitivity of the biosensor. | es_ES |
dc.description.sponsorship | The authors acknowledge the financial support from the projects European Communities Seventh Framework Program: SEA-on-a-CHIP (FP7-OCEAN-2013) under the grant agreement N° 614168, and funding from the European Union’s Horizon 2020 research and innovation program entitled KardiaTool under grant agreement N° 643694, and the fellowship from Science Without Borders program—Conselho Nacional de Desenvolvimento Ciêntífico Tenológico (CNPq)–Brazil grant N° 249056/2013-5. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Biosensors | es_ES |
dc.subject | Sulfapyridine | es_ES |
dc.subject | SA2BSA | es_ES |
dc.subject | BioMEMS | es_ES |
dc.subject | Magnetic nanoparticles | es_ES |
dc.subject | Competitive assay | es_ES |
dc.title | Development of Novel Magneto-Biosensor for Sulfapyridine Detection | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.3390/bios10040043 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/bios10040043 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.contributor.orcid | Salvador, Juan Pablo [0000-0002-3608-0634] | es_ES |
dc.contributor.orcid | Marco, María Pilar [0000-0002-2237-7197] | es_ES |
dc.identifier.pmid | 32326302 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Development of Novel Magneto-Biosensor for Sulfapyridine Detection.pdf | Artículo principal | 1,52 MB | Adobe PDF | Visualizar/Abrir |
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