Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/17887
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Wound-induced phenolic accumulation and browning in lettuce (Lactuca sativa L.) leaf tissue is reduced by exposure to n-alcohols

AuthorsTomás Barberán, Francisco CSIC ORCID ; Choi, Young-Jun; Saltveit, Mikal E.
KeywordsAbiotic stress
Fresh-cut
Jasmonic acid
Octadecanoid pathway
Oxylipin pathway
Phospholipases D
Wounding
Issue DateJul-2005
PublisherElsevier
CitationPostharvest Biology and Technology 37(1): 47-55 (2005)
AbstractA wound signal originates at the site of injury in lettuce (Lactuca sativa L.) leaf tissue and propagates into adjacent tissue where it induces a number of physiological responses which include increased phenolic metabolism with the de novo synthesis of phenylalanine ammonia lyase (PAL, EC 4.3.1.5), the synthesis and accumulation of soluble phenolic compounds (e.g., chlorogenic acid), and subsequent tissue browning. Exposing excised mid-rib leaf tissue to vapors (20 μmol/(g FW)) or aqueous solutions (100 mM) of n-alcohols inhibited this wound-induced tissue browning by 40 and 60%, respectively. Effectiveness of the alcohol increased linearly from ethanol to the 7-carbon heptanol, and then was lost for the longer n-alcohols 1-octanol and 1-nonanol. The 2- and 3-isomers of the effective alcohols did not significantly reduce wound-induced phenolic accumulation at optimal 1-alcohol concentrations, but significant reductions did occur at much higher concentrations (100 μmol/(g FW)) of the 2-, and 3-isomers. The active n-alcohols were maximally effective when applied during the first 2 h after excision, and were ineffective if applied 6 h after excision. Phospholipase D (PLD) and its products linolenic acid (LA) and phosphatidic acid (PA) are thought to initiate the oxylipin pathway that culminates in the production of jasmonic acid, and PLD is specifically inhibited by 1-butanol, but not by 2-, or 3-butanol. These results suggest that PLD, LA, PA, and the oxylipin pathway may be involved in producing the wound signal responsible for increased wound-induced PAL activity, phenolic accumulation and browning in fresh-cut lettuce leaf tissue.
Description9 pages, 7 figures.
Publisher version (URL)http://dx.doi.org/10.1016/j.postharvbio.2005.03.002
URIhttp://hdl.handle.net/10261/17887
DOI10.1016/j.postharvbio.2005.03.002
ISSN0925-5214
Appears in Collections:(CEBAS) Artículos

Show full item record
Review this work

SCOPUSTM   
Citations

73
checked on May 12, 2022

WEB OF SCIENCETM
Citations

71
checked on May 15, 2022

Page view(s)

353
checked on May 16, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.