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Título: | RNA-protein interaction methods to study viral IRES elements |
Autor: | Francisco-Velilla, Rosario CSIC ORCID; Fernández-Chamorro, Javier; Lozano, Gloria; Díaz-Toledano, Rosa CSIC; Martínez-Salas, Encarnación CSIC ORCID | Palabras clave: | RNA virus Translation control RNA structure RNA-binding proteins IRES elements |
Fecha de publicación: | 15-feb-2015 | Editor: | Academic Press | Citación: | Methods 91: 3- 12 (2015) | Resumen: | Translation control often takes place through the mRNA untranslated regions, involving direct interactions with RNA-binding proteins (RBPs). Internal ribosome entry site elements (IRESs) are cis-acting RNA regions that promote translation initiation using a cap-independent mechanism. A subset of positive-strand RNA viruses harbor IRESs as a strategy to ensure efficient viral protein synthesis. IRESs are organized in modular structural domains with a division of functions. However, viral IRESs vary in nucleotide sequence, secondary RNA structure, and transacting factor requirements. Therefore, in-depth studies are needed to understand how distinct types of viral IRESs perform their function. In this review we describe methods to isolate and identify RNA-binding proteins important for IRES activity, and to study the impact of RNA structure and RNA-protein interactions on IRES activity. | URI: | http://hdl.handle.net/10261/140262 | DOI: | 10.1016/j.ymeth.2015.06.023 | Identificadores: | doi: 10.1016/j.ymeth.2015.06.023 issn: 1095-9130 |
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Martínez-SalasE_RNA-ProteinInteractionMethods.pdf | 1,3 MB | Adobe PDF | Visualizar/Abrir |
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