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RNA-protein interaction methods to study viral IRES elements

AuthorsFrancisco-Velilla, Rosario ; Fernández-Chamorro, Javier; Lozano, Gloria; Díaz-Toledano, Rosa ; Martínez-Salas, Encarnación
KeywordsRNA virus
Translation control
RNA structure
RNA-binding proteins
IRES elements
Issue Date15-Feb-2015
PublisherAcademic Press
CitationMethods 91: 3- 12 (2015)
AbstractTranslation control often takes place through the mRNA untranslated regions, involving direct interactions with RNA-binding proteins (RBPs). Internal ribosome entry site elements (IRESs) are cis-acting RNA regions that promote translation initiation using a cap-independent mechanism. A subset of positive-strand RNA viruses harbor IRESs as a strategy to ensure efficient viral protein synthesis. IRESs are organized in modular structural domains with a division of functions. However, viral IRESs vary in nucleotide sequence, secondary RNA structure, and transacting factor requirements. Therefore, in-depth studies are needed to understand how distinct types of viral IRESs perform their function. In this review we describe methods to isolate and identify RNA-binding proteins important for IRES activity, and to study the impact of RNA structure and RNA-protein interactions on IRES activity.
Identifiersdoi: 10.1016/j.ymeth.2015.06.023
issn: 1095-9130
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