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dc.contributor.authorFrancisco-Velilla, Rosario-
dc.contributor.authorFernández-Chamorro, Javier-
dc.contributor.authorLozano, Gloria-
dc.contributor.authorDíaz-Toledano, Rosa-
dc.contributor.authorMartínez-Salas, Encarnación-
dc.date.accessioned2016-11-16T08:38:48Z-
dc.date.available2016-11-16T08:38:48Z-
dc.date.issued2015-02-15-
dc.identifierdoi: 10.1016/j.ymeth.2015.06.023-
dc.identifierissn: 1095-9130-
dc.identifier.citationMethods 91: 3- 12 (2015)-
dc.identifier.urihttp://hdl.handle.net/10261/140262-
dc.description.abstractTranslation control often takes place through the mRNA untranslated regions, involving direct interactions with RNA-binding proteins (RBPs). Internal ribosome entry site elements (IRESs) are cis-acting RNA regions that promote translation initiation using a cap-independent mechanism. A subset of positive-strand RNA viruses harbor IRESs as a strategy to ensure efficient viral protein synthesis. IRESs are organized in modular structural domains with a division of functions. However, viral IRESs vary in nucleotide sequence, secondary RNA structure, and transacting factor requirements. Therefore, in-depth studies are needed to understand how distinct types of viral IRESs perform their function. In this review we describe methods to isolate and identify RNA-binding proteins important for IRES activity, and to study the impact of RNA structure and RNA-protein interactions on IRES activity.-
dc.description.sponsorshipBFU2011-25437 and CSD2009-00080 from MINECO (Ministerio de Economia y Competitividad), and by an Institutional grant from Fundación Ramón Areces.-
dc.publisherAcademic Press-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.subjectRNA virus-
dc.subjectTranslation control-
dc.subjectRNA structure-
dc.subjectRNA-binding proteins-
dc.subjectIRES elements-
dc.titleRNA-protein interaction methods to study viral IRES elements-
dc.typeartículo-
dc.identifier.doi10.1016/j.ymeth.2015.06.023-
dc.date.updated2016-11-16T08:38:49Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderFundación Ramón Areces-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/100008054es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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