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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Merkoçi, Arben | es_ES |
dc.contributor.author | Aldavert, Marta | es_ES |
dc.contributor.author | Tarrasón, Gema | es_ES |
dc.contributor.author | Eritja Casadellà, Ramón | es_ES |
dc.contributor.author | Alegret, Salvador | es_ES |
dc.date.accessioned | 2016-09-13T07:12:46Z | - |
dc.date.available | 2016-09-13T07:12:46Z | - |
dc.date.issued | 2005-10-01 | - |
dc.identifier.citation | Analytical Chemistry - Columbus 77(19): 6500-6503 (2005) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/136645 | - |
dc.description.abstract | Gold nanoparticles modified with anti-mouse IgG have been used to trace oligonucleotides carrying a c-myc peptide. Two strategies, a dot-blot format as well as inductively coupled plasma mass spectrometry (ICPMS) have been used to detect the nanoparticle tracer. For both cases, oligonucleotide-peptide conjugates were first applied to a nitrocellulose membrane using a manifold attached to a suction device. After immobilization of the oligonucleotide by UV radiation, the samples were incubated with an anti-c-myc monoclonal antibody. In the case of the dot-blot format strategy, it was followed by incubation with a secondary antibody conjugated to horseradish peroxidase and development with luminol as chemiluminescent substrate. In the case of ICPMS strategy, it was followed by incubation with the secondary antibody (anti-mouse IgG) conjugated to gold nanoparticles and their ICPMS detection after dissolving. The nonspecific adsorptions were found to be around zero. The limit of detection for peptide-modified DNA was 0,2 pmol. The method may have significant potential as an important ICPMS-based nonradioactive DNA detection method for the simultaneous determination of various sequences by labeling different kinds of inorganic nanoparticles. © 2005 American Chemical Society. | es_ES |
dc.description.sponsorship | This work was financially supported from MEC (Madrid) (Projects BIO2004- 02776, MAT2004-05164), by Spanish foundation “Ramón Areces” (project ‘Bionanosensores’). A.M. thanks the “Ramón y Cajal” program of Ministry of Science and Technology (Madrid). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.isversionof | Postprint | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Gold nanoparticles | es_ES |
dc.subject | Inductively coupled plasma mass spectrometry (ICPMS) | es_ES |
dc.subject | DNA | es_ES |
dc.subject | Atomic emission spectrometry | es_ES |
dc.title | Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1021/ac050539l | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1021/ac050539l | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Merkoci2005AnalChem.pdf | 217,47 kB | Adobe PDF | Visualizar/Abrir |
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