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dc.contributor.authorMerkoçi, Arbenes_ES
dc.contributor.authorAldavert, Martaes_ES
dc.contributor.authorTarrasón, Gemaes_ES
dc.contributor.authorEritja Casadellà, Ramónes_ES
dc.contributor.authorAlegret, Salvadores_ES
dc.identifier.citationAnalytical Chemistry - Columbus 77(19): 6500-6503 (2005)es_ES
dc.description.abstractGold nanoparticles modified with anti-mouse IgG have been used to trace oligonucleotides carrying a c-myc peptide. Two strategies, a dot-blot format as well as inductively coupled plasma mass spectrometry (ICPMS) have been used to detect the nanoparticle tracer. For both cases, oligonucleotide-peptide conjugates were first applied to a nitrocellulose membrane using a manifold attached to a suction device. After immobilization of the oligonucleotide by UV radiation, the samples were incubated with an anti-c-myc monoclonal antibody. In the case of the dot-blot format strategy, it was followed by incubation with a secondary antibody conjugated to horseradish peroxidase and development with luminol as chemiluminescent substrate. In the case of ICPMS strategy, it was followed by incubation with the secondary antibody (anti-mouse IgG) conjugated to gold nanoparticles and their ICPMS detection after dissolving. The nonspecific adsorptions were found to be around zero. The limit of detection for peptide-modified DNA was 0,2 pmol. The method may have significant potential as an important ICPMS-based nonradioactive DNA detection method for the simultaneous determination of various sequences by labeling different kinds of inorganic nanoparticles. © 2005 American Chemical Society.es_ES
dc.description.sponsorshipThis work was financially supported from MEC (Madrid) (Projects BIO2004- 02776, MAT2004-05164), by Spanish foundation “Ramón Areces” (project ‘Bionanosensores’). A.M. thanks the “Ramón y Cajal” program of Ministry of Science and Technology (Madrid).es_ES
dc.publisherAmerican Chemical Societyes_ES
dc.subjectGold nanoparticleses_ES
dc.subjectInductively coupled plasma mass spectrometry (ICPMS)es_ES
dc.subjectAtomic emission spectrometryes_ES
dc.titleToward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequenceses_ES
dc.description.peerreviewedPeer reviewedes_ES
oprm.item.hasRevisionno ko 0 false*
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