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http://hdl.handle.net/10261/13424
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Parro-García, Víctor | en_US |
dc.contributor.author | Moreno-Paz, Mercedes | en_US |
dc.contributor.author | González-Toril, Elena | en_US |
dc.date.accessioned | 2009-06-03T05:56:10Z | - |
dc.date.available | 2009-06-03T05:56:10Z | - |
dc.date.issued | 2007-02 | en_US |
dc.identifier.citation | Environmental Microbiology 9(2): 453-464 (2007) | en_US |
dc.identifier.issn | 1462-2912 | - |
dc.identifier.uri | http://hdl.handle.net/10261/13424 | - |
dc.description | 12 pages, 4 figures, 1 table.-- ISI Article Identifier: 000243376800017.-- Available online Oct 25, 2006. | en_US |
dc.description | GenBank accession numbers for more than 100 000 nt of new sequences: DQ785317–DQ785468. | - |
dc.description | Supporting information (Suppl. figs S1-S4, tables S1-S2, appendix S1) available at: http://www3.interscience.wiley.com/journal/118491134/suppinfo | - |
dc.description.abstract | In this work we investigated the correlations between global gene expression patterns and environmental parameters in natural ecosystems. We studied the preferential gene expression of the iron oxidizer bacterium Leptospirillum ferrooxidans to adapt its physiology to changes in the physicochemical parameters in its natural medium. Transcriptome analysis by DNA microarrays can proportionate an instant picture about the preferential gene expression between two different environmental samples. However, this type of analysis is very difficult and complex in natural ecosystems, mainly because of the broad biodiversity and multiple environmental parameters that may affect gene expression. The necessity of high-quality RNA preparations as well as complicated data analysis are also technological limitations. The low prokaryotic diversity of the extremely acidic and iron-rich waters of the Tinto River (Spain) ecosystem, where L. ferrooxidans is abundant, allows the opportunity to achieve global gene expression studies and to associate gene function with environmental parameters. We applied a total RNA amplification protocol validated previously for the amplification of the environmental transcriptome (meta-transcriptome). The meta-transcriptome of two sites from the Tinto River mainly differing in the salt and oxygen contents were amplified and analysed by a L. ferrooxidans DNA microarray. The results showed a clear preferential induction of genes involved in certain physicochemical parameters like: high salinity (ectAB, otsAB), low oxygen concentration (cydAB), iron uptake (fecA-exbBD-tonB), oxidative stress (carotenoid synthesis, oxyR, recG), potassium (kdpBAC) or phosphate concentrations (pstSCAB), etc. We conclude that specific gene expression patterns can be useful indicators for the physiological conditions in a defined ecosystem. Also, the upregulation of certain genes and operons reveals information about the environmental conditions (nutrient limitations, stresses, etc.). | - |
dc.description.sponsorship | This work was supported by the Centro de Astrobiología internal budget and is part of a ‘Ramón y Cajal’ project. Dr. Victor Parro has a ‘Ramón y Cajal’ contract from Ministerio de Educación y Ciencia at the Instituto Nacional de Técnica Aeroespacial ‘Esteban Terradas’ (INTA); Mercedes Moreno-Paz and Marina Postigo are personnel from INTA; and Miriam García has a grant from INTA. | - |
dc.format.extent | 2373 bytes | - |
dc.format.mimetype | text/plain | - |
dc.publisher | Society for Applied Microbiology | - |
dc.publisher | Blackwell Publishing | - |
dc.rights | closedAccess | en_US |
dc.title | Analysis of environmental transcriptomes by DNA microarrays | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1111/j.1462-2920.2006.01162.x | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1111/j.1462-2920.2006.01162.x | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | CSIC-INTA - Centro de Astrobiología (CAB) | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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