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Title

Type-f thioredoxins have a role in the short-term activation of carbon metabolism and their loss affects growth under short-day conditions in Arabidopsis thaliana

AuthorsNaranjo, Belén ; Díaz-Espejo, Antonio ; Lindahl, Marika ; Cejudo, Francisco Javier
Keywords6 bisphosphatase
Carbon assimilation
Chloroplast
Fructose 1
Photosynthesis
Redox regulation
Thioredoxin
Issue Date2-Feb-2016
PublisherOxford University Press
CitationJournal of Experimental Botany 67(6): 1951-1964 (2016)
AbstractRedox regulation plays a central role in the adaptation of chloroplast metabolism to light. Extensive biochemical analyses in vitro have identified f-type thioredoxins (Trxs) as the most important catalysts for light-dependent reduction and activation of the enzymes of the Calvin-Benson cycle. However, the precise function of type f Trxs in vivo and their impact on plant growth are still poorly known. To address this issue we have generated an Arabidopsis thaliana double knock-out mutant, termed trxf1f2, devoid of both f1 and f2 Trxs. Despite the essential function previously proposed for f-type Trxs, the visible phenotype of the trxf1f2 double mutant was virtually indistinguishable from the wild type when grown under a long-day photoperiod. However, the Trx f-deficient plants showed growth inhibition under a short-day photoperiod which was not rescued at high light intensity. The absence of f-type Trxs led to significantly lower photosynthetic electron transport rates and higher levels of non-photochemical energy quenching. Notably, the Trx f null mutant suffered from a shortage of photosystem I electron acceptors and delayed activation of carbon dioxide fixation following a dark-light transition. Two redox-regulated Calvin-Benson cycle enzymes, fructose 1,6-bisphosphatase (FBPase) and Rubisco activase, showed retarded and incomplete reduction in the double mutant upon illumination, compared with wild-type plants. These results show that the function of f-type Trxs in the rapid activation of carbon metabolism in response to light is not entirely compensated for by additional plastid redox systems, and suggest that these Trxs have an important role in the light adjustment of photosynthetic metabolism.
Description14 páginas.-- 11 figuras.-- 57 referencias.-- Supplementary data can be found at JXB online http://jxb.oxfordjournals.org/content/early/2016/02/02/jxb.erw017/suppl/DC1
Publisher version (URL)http://dx.doi.org/10.1093/jxb/erw017
URIhttp://hdl.handle.net/10261/131651
DOI10.1093/jxb/erw017
ISSN0022-0957
Appears in Collections:(IRNAS) Artículos
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