Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/126550
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Bernet Zamanillo, Gillermo P. | es_ES |
dc.contributor.author | Elena, Santiago F. | es_ES |
dc.date.accessioned | 2015-12-08T11:49:25Z | - |
dc.date.available | 2015-12-08T11:49:25Z | - |
dc.date.issued | 2015-12-08 | - |
dc.identifier.citation | BMC Evolutionary Biology 15: 274 (2015) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/126550 | - |
dc.description.abstract | [Background[ Understanding the causes and consequences of phenotypic variability is a central topic of evolutionary biology. Mutations within non-coding cis-regulatory regions are thought to be of major effect since they affect the expression of downstream genes. To address the evolutionary potential of mutations affecting such regions in RNA viruses, we explored the fitness properties of mutations affecting the 5’-untranslated region (UTR) of a prototypical member of the picorna-like superfamily, tobacco etch virus (TEV). This 5’ UTR acts as an internal ribosomal entry site (IRES) and is essential for expression of all viral genes. | es_ES |
dc.description.abstract | [Results] We determined in vitro the folding of 5’ UTR using the selective 2’-hydroxyl acylation analyzed by primer extension (SHAPE) technique. Then, we created a collection of single-nucleotide substitutions on this region and evaluated the statistical properties of their fitness effects in vivo. We found that, compared to random mutations affecting coding sequences, mutations at the 5’ UTR were of weaker effect. We also created double mutants by combining pairs of these single mutations and found variation in the magnitude and sign of epistatic interactions, with an enrichment of cases of positive epistasis. A correlation exists between the magnitude of fitness effects and the size of the perturbation made in the RNA folding structure, suggesting that the larger the departure from the predicted fold, the more negative impact in viral fitness. | - |
dc.description.abstract | [Conclusions] Evidence that mutational fitness effects on the short 5’ UTR regulatory sequence of TEV are weaker than those affecting its coding sequences have been found. Epistasis among pairs of mutations on the 5’ UTR ranged between the extreme cases of synthetic lethal and compensatory. A plausible hypothesis to explain all these observations is that the interaction between the 5’ UTR and the host translational machinery was shaped by natural selection to be robust to mutations, thus ensuring the homeostatic expression of viral genes even at high mutation rates. | - |
dc.description.sponsorship | This work was supported by grant BFU2012-30805 from the Spanish Ministry of Economy and Competitiveness (MINECO), grant PROMETEOII/2014/021 from Generalitat Valenciana and the EvoEvo (ICT610427) project from the European Commission 7th Framework Program. Publication fees have been partially paid by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/610427 | - |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Distribution of mutational fitness effects | es_ES |
dc.subject | Plant virus | es_ES |
dc.subject | Potyvirus | es_ES |
dc.subject | RNA regulatory sequences | es_ES |
dc.title | Distribution of mutational fitness effects and of epistasis in the 5' untranslated region of a plant RNA virus | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1186/s12862-015-0555-2 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1186/s12862-015-0555-2 | es_ES |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Generalitat Valenciana | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI) | - |
dc.relation.csic | Sí | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003359 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.pmid | 26643527 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IBMCP) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
s12862-015-0555-2 (1).pdf | 858,73 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
8
checked on 13-may-2024
SCOPUSTM
Citations
10
checked on 09-may-2024
WEB OF SCIENCETM
Citations
11
checked on 26-feb-2024
Page view(s)
387
checked on 12-may-2024
Download(s)
254
checked on 12-may-2024