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Título: | Identification of commercial hake and grenadier species by proteomic analysis of the parvalbumin fraction |
Autor: | Carrera, Mónica CSIC ORCID ; Cañas, Benito; Piñeiro, Carmen CSIC ORCID; Vázquez, Jesús CSIC ORCID CVN; Gallardo, José Manuel CSIC | Palabras clave: | Fish identification MALDI-TOF MS Merlucciidae Parvalbumin |
Fecha de publicación: | 23-ago-2006 | Editor: | John Wiley & Sons | Citación: | Proteomics 6(19): 5278-5287 (2006) | Resumen: | Analysis of parvalbumin fractions through proteomic methodologies allowed the differential classification of ten commercial, closely related species of the family Merlucciidae. Muscle extracts from nine hake species of the genus Merluccius including two subspecies of Merluccius australis (australis and polylepsis) and one grenadier species Macruronus novaezelandiae with two populations (novaezelandiae and magellanicus) were evaluated by 2-DE and MALDI-TOF MS. 2-DE demonstrated that the species tested displayed a low intra-specific degree of polymorphism and the isoform patterns were noticeably species-specific. MALDI-TOF mass fingerprints showed clear differences in the pattern of peptides produced by tryptic digestion between the Merluccius and the Macruronus, making the genus differentiation possible. In addition, a selective peptide mass present in the spectra from certain hakes allowed its classification in two groups: Euro-African and American hakes. Besides, some specific masses allowed a clearly individual identification for M. bilinearis, M. australis polylepsis, M. australis australis, M. productus, M. paradoxus and M. polli, while the rest of the hake species can be grouped in two clusters, comprising M. hubbsi and M. gayi in one and M. merluccius and M. capensis in the other. | Descripción: | 10 pages, 3 figures.-- PMID: 16927426 [PubMed].-- Supplementary information (Results and discussion, figure S1, tables S1-S5, 8 pages) available at: http://www.wiley-vch.de/contents/jc_2120/2006/pro200500899_s.pdf Printed version published Oct 2006. |
Versión del editor: | http://dx.doi.org/10.1002/pmic.200500899 | URI: | http://hdl.handle.net/10261/10156 | DOI: | 10.1002/pmic.200500899 | ISSN: | 1615-9853 |
Aparece en las colecciones: | (IIM) Artículos (CBM) Artículos |
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