Raman spectroscopy study of the structural effect of microbial transglutaminase on meat systems and its relationship with textural characteristics

Abstract

Raman spectroscopy and texture analysis (TPA) studies were carried out to determine the effect of adding different levels of microbial transglutaminase (MTGase) to meat systems. This addition produced a significant (p < 0.05) increase in hardness, springiness and cohesiveness in the meat systems. Raman spectroscopy analysis revealed the occurrence of secondary structural changes in meat proteins due to MTGase. Modifications in the amide I (1650-1680 cm-1) and amide III (1200-1300 cm-1) regions indicated a significant (p < 0.05) decrease in α-helix content, accompanied by a significant (p < 0.05) increase in β-sheets and turns due to the addition of the enzyme to meat systems. Significant (p < 0.05) correlations were found between these secondary structural changes in meat proteins and the textural properties (hardness, adhesiveness, springiness and cohesiveness) of meat systems. © 2007 Elsevier Ltd. All rights reserved.