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http://hdl.handle.net/10261/89534
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dc.contributor.author | Rojano-Delgado, Antonia María | - |
dc.contributor.author | Priego Capote, Feliciano | - |
dc.contributor.author | Barro Losada, Francisco | - |
dc.contributor.author | Luque de Castro, María Dolores | - |
dc.contributor.author | Prado, R. del | - |
dc.date.accessioned | 2014-01-16T08:22:06Z | - |
dc.date.available | 2014-01-16T08:22:06Z | - |
dc.date.issued | 2013-12 | - |
dc.identifier | doi: 10.1016/j.phytochem.2013.10.008 | - |
dc.identifier | issn: 0031-9422 | - |
dc.identifier.citation | Phytochemistry 96: 117-122 (2013) | - |
dc.identifier.uri | http://hdl.handle.net/10261/89534 | - |
dc.description.abstract | The resistance to glufosinate of two lines - genetically modified (GM) and unmodified (T-590 and T-549, respectively) - of Triticum aestivum has been studied. In the GM line, the bar gene was introduced to increase the resistance to glufosinate. Experiments in a controlled growth chamber showed that line T-590 presented a high resistance to glufosinate with an ED50 value of 478.59 g active ingredient per hectare (g ai ha-1) versus 32.65 g ai ha-1 for line T-549. The activity of glutamine synthetase (GS) in leaf extracts from both lines was investigated. The I50 for line T-590 was 694.10 μM glufosinate versus 55.46 μM for line T-549, with a resistance factor of 12.51. Metabolism studies showed a higher and faster penetration of glufosinate in line T-549 than in line T-590. LC-TOF/MS analysis of glufosinate metabolism at 48 h after herbicide treatment (300 g ai ha -1) revealed an 83.4% conversion of the herbicide (66.5% in N-acetyl-glufosinate metabolite), while in line T-549 conversion of the herbicide was about 40% (0% to N-acetyl-glufosinate). These results suggest that metabolism of glufosinate by the bar gene is a key mechanism of resistance in line T-590 that explains such high levels of herbicide tolerated by the plant, together with other mechanisms due to unmodified pathway, absorption and loss of glufosinate affinity for its target site.© 2013 Elsevier Ltd. All rights reserved. | - |
dc.description.sponsorship | The authors thank to the Spanish Ministerio de Ciencia e Innovación (MICINN) and FEDER program for financial support through Projects No. AGL-2010 16774, CTM2009-07430 and CTQ2012-37428. F.P.C. is also grateful to the MICINN for a Ramón y Cajal contract (RYC-2009-03921). | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.rights | closedAccess | - |
dc.subject | Triticum aestivum | - |
dc.subject | GMO crops | - |
dc.subject | Glufosinate | - |
dc.subject | Resistance | - |
dc.subject | Metabolism | - |
dc.subject | Degradation | - |
dc.subject | LC–DAD | - |
dc.subject | LC–TOF/MS | - |
dc.title | Liquid chromatography-diode array detection to study the metabolism of glufosinate in Triticum aestivum T-590 and influence of the genetic modification on its resistance | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.phytochem.2013.10.008 | - |
dc.date.updated | 2014-01-16T08:22:06Z | - |
dc.description.version | Peer Reviewed | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
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