Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/59327
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The influence of substrate structure on the kinetics of the hydrolysis of starch by glucoamylase |
Autor: | Lema, J. M.; Sanromán, A.; Murado García, Miguel Anxo | Fecha de publicación: | 1996 | Editor: | Humana Press | Citación: | Applied Biochemistry and Biotechnology. 60(2): 329-336 (1996) | Resumen: | In this research the influence of substrate structure on the kinetics of the enzymatic hydrolysis of starch by glucoamylase was evaluated. For this purpose, two substrates of different form and molecular weight were used. In one case, the kinetics of the hydrolysis corresponds to a typical Michaelis-Menten behavior; in the other, a decrease of the hydrolysis rate occurred once a determined substrate concentration was surpassed. The structural differences between the starches, which caused important differences on the rheological properties of their solutions, justify the observed differences in their behavior. Branching of the substrate exerts two opposite effects on the hydrolysis rate because it allows the increase of the number of available points for the enzymatic attack, although the branching increases the steric hindrances and, consequently, the mass transfer resistances. The balance between these two effects is clearly dependent on the substrate concentration. | Descripción: | 8 páginas | URI: | http://hdl.handle.net/10261/59327 | ISSN: | 0273-2289 | E-ISSN: | 1559-0291 |
Aparece en las colecciones: | (IIM) Artículos |
Mostrar el registro completo
CORE Recommender
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.