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Title: | φ29 DNA polymerase |
Authors: | Blanco, Luis CSIC ORCID ; Bernad, Antonio CSIC ORCID; Salas, Margarita CSIC ORCID | Issue Date: | 19-Nov-1996 | Citation: | Publicación nr.: US 5576204 | Abstract: | An improved method for determining the nucleotide base sequence of a DNA molecule employs a φ-29 type DNA polymerase modified to have reduced or no exonuclease activity. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a φ29-type DNA polymerase. | Description: | Titular: Consejo Superior Investigaciones Cientificas (CSIC) | Publisher version (URL): | http://www.patentlens.net/patentlens/patents.html?patnums=US_5576204_A#tab_1 | URI: | http://hdl.handle.net/10261/39772 |
Appears in Collections: | (CBM) Patentes |
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5576204_phi_29_DNA_polymerase (1).pdf | 237,72 kB | Adobe PDF | View/Open |
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