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http://hdl.handle.net/10261/39772
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Blanco, Luis | - |
dc.contributor.author | Bernad, Antonio | - |
dc.contributor.author | Salas, Margarita | - |
dc.date.accessioned | 2011-09-21T09:08:04Z | - |
dc.date.available | 2011-09-21T09:08:04Z | - |
dc.date.issued | 1996-11-19 | - |
dc.identifier.citation | Publicación nr.: US 5576204 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/39772 | - |
dc.description | Titular: Consejo Superior Investigaciones Cientificas (CSIC) | es_ES |
dc.description.abstract | An improved method for determining the nucleotide base sequence of a DNA molecule employs a φ-29 type DNA polymerase modified to have reduced or no exonuclease activity. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a φ29-type DNA polymerase. | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | openAccess | es_ES |
dc.title | φ29 DNA polymerase | es_ES |
dc.type | patente | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://www.patentlens.net/patentlens/patents.html?patnums=US_5576204_A#tab_1 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_15cd | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | patente | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
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5576204_phi_29_DNA_polymerase (1).pdf | 237,72 kB | Adobe PDF | Visualizar/Abrir |
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