English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/38206
Compartir / Impacto:
Título : Peroxiredoxins and NADPH-Dependent Thioredoxin Systems in the Model Legume Lotus japonicus
Autor : Tovar-Méndez, Alejandro ; Matamoros Galindo, Manuel Ángel ; Bustos-Sanmamed, Pilar ; Dietz, Karl-Josef; Cejudo, Francisco Javier ; Rouhier, Nicolas; Sato, Shusei; Tabata, Satoshi; Becana Ausejo, Manuel
Fecha de publicación : jul-2011
Editor: American Society of Plant Biologists
Citación : Tovar-Méndez A, Matamoros MA, Bustos-Sanmamed P, Dietz KJ, Cejudo FJ, Rouhier N, Sato S, Tabata S, Becana M. Peroxiredoxins and NADPH-Dependent Thioredoxin Systems in the Model Legume Lotus japonicus. Plant physiology 156 (3): 1535-1547 (2011)
Resumen: Peroxiredoxins (Prxs), thioredoxins (Trxs), and NADPH-thioredoxin reductases (NTRs) constitute central elements of the thiol-disulfide redox regulatory network of plant cells. This study provides a comprehensive survey of this network in the model legume Lotus japonicus. The aims were to identify and characterize these gene families and to assess whether the NTR-Trx systems are operative in nodules. Quantitative reverse transcription-polymerase chain reaction and immunological and proteomic approaches were used for expression profiling. We identified seven Prx, 14 Trx, and three NTR functional genes. The PrxQ1 gene was found to be transcribed in two alternative spliced variants and to be expressed at high levels in leaves, stems, petals, pods, and seeds and at low levels in roots and nodules. The 1CPrx gene showed very high expression in the seed embryos and low expression in vegetative tissues and was induced by nitric oxide and cytokinins. In sharp contrast, cytokinins down-regulated all other Prx genes, except PrxQ1, in roots and nodules, but only 2CPrxA and PrxQ1 in leaves. Gene-specific changes in Prx expression were also observed in response to ethylene, abscisic acid, and auxins. Nodules contain significant mRNA and protein amounts of cytosolic PrxIIB, Trxh1, and NTRA and of plastidic NTRC. Likewise, they express cytosolic Trxh3, Trxh4, Trxh8, and Trxh9, mitochondrial PrxIIF and Trxo, and plastidic Trxm2, Trxm4, and ferredoxin-Trx reductase. These findings reveal a complex regulation of Prxs that is dependent on the isoform, tissue, and signaling molecule and support that redox NTR-Trx systems are functional in the cytosol, mitochondria, and plastids of nodules.
Descripción : 13 Pag., 7 Fig., 1 Tabl.
Versión del editor: http://dx.doi.org/10.1104/pp.111.177196
URI : http://hdl.handle.net/10261/38206
DOI: 10.1104/pp.111.177196
ISSN: 0032-0889
E-ISSN: 1532-2548
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BecanaM_PlantPhhys_2011.pdf46,17 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.