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Título: | Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis |
Autor: | Medeot, Daniela; Sannazzaro, Analia; Estrella, María Julia; Torres Tejerizo, Gonzalo; Contreras-Moreira, Bruno CSIC ORCID ; Pistorio, Mariano; Jofré, Edgardo | Palabras clave: | Applied microbiology Microbial genetics Microbiology |
Tesauro UNESCO: | Microbiology | Tesauro AGROVOC: | soil bacteria bacterial genomes genetic engineering |
Fecha de publicación: | dic-2023 | Editor: | Nature Publishing Group | Citación: | Medeot D, Sannazzaro D, Estrella MJ, Torres G, Contreras-Moreira B, Pistorio M, Jofré E. Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis. Scientific Reports 13: 22168 (2023) | Resumen: | Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16–C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892 bp, with a total of 3474 coding sequences and a G + C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1–4 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16–C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes. | Descripción: | 14 Pags.- 8 Figs.- 1 Tabl. Data generated in this research are provided as supplementary material with this manuscript. DNA sequence data are deposited in the NCBI GenBank database under the accession number CP042864.2. © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, | Versión del editor: | https://doi.org/10.1038/s41598-023-49194-y | URI: | http://hdl.handle.net/10261/355252 | DOI: | 10.1038/s41598-023-49194-y | E-ISSN: | 2045-2322 |
Aparece en las colecciones: | (EEAD) Artículos |
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