Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and signalling pathways related to proliferation and differentiation in early embryo development through post-transcriptional mechanisms. A previous study showed that bta-mir 483-3p was exclusively detected in extracellular vesicles from the oviductal fluid of pregnant cows compared to non-pregnant (Mazzarella et al., 2021). Therefore, we aimed to determine whether bta-miR-483-3p is uptaken by bovine in vitro-produced embryos through passive transfection (gymnosis) and to evaluate the effect of miRNA supplementation on early embryo development and quality.